To study in vitro the possible genotoxic and/or co-genotoxic activity of 50 Hz magnetic fields by using the comet assay.
Sets of experiments were performed to evaluate the possible interaction between 50 Hz magnetic fields and the known leukemogen benzene. Three benzene hydroxylated metabolites were also evaluated: 1,2-benzenediol (catechol), 1,4-benzenediol (hydroquinone), and 1,2,4-benzenetriol.
Exposure duration: continuous for 1 h
Experiment comprised of following groups: Jürkat cells 1) exposed to MF, 2) sham exposed, 3) neither exposed nor sham exposed, 4) exposed to MF and xenobiotics, 5) sham exposed and exposed to xenobiotics, 6) not exposed to MF but exposed to xenobiotics.
|Exposure duration||continuous for 1 h|
|Chamber||CO2 incubator, with the Helmholtz coil system placed in it, maintained at 37°C|
|Setup||the magnetic field was oriented in the plane of the cell culture plates|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||1 mT||peak value||measured||-||-|
Cells co-exposed to 1mT magnetic fields, benzene and catechol did not show any genotoxic activity. However, co-exposure of cells to 1mT magnetic fields and hydroquinone led to the appearance of a clear genotoxic effect. Moreover, co-exposure of cells to 1mT magnetic fields and 1,2,4-benzenetriol led to a marked increase in the genotoxicity of the ultimate metabolite of benzene.
The possibility that 50 Hz (power frequency) magnetic fields might interfere with the genotoxic activity of xenobiotics has important implications, since humans are likely to be exposed to a variety of genotoxic agents concomitantly with exposure to this type of physical agent.
Remark: The authors speak about Jürkat cells. It is supposed that they mean Jurkat cells.