To examine the effects of 50 Hz magnetic fields on DNA damage in wild type embryonic mouse fibroblasts and in mutated (Atm-deficient) mouse embryonic fibroblasts.
In humans, ataxia telangiectasia (or Louis-Bar syndrome) is a rare autosomal recessive recessive disease caused by a mutation in the Atm-gene, which plays a central role in double-strand break repair. In research, Atm-deficient cells are used as a model to investigate genotoxic agents because they are extremely sensitive.
A positive control was conducted with 0.1 mM 4-nitroquinoline-1-oxide.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
15 minutes, 1 hour or 24 hours
genotoxicity tests and cell cycle distribution
|
|
|
Exposure 2:
50 Hz
Exposure duration:
1 hour or 24 hours
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 15 minutes, 1 hour or 24 hours |
| Additional info | genotoxicity tests and cell cycle distribution |
| Exposure source |
|
|---|---|
| Chamber | cells were cultured and exposed in a dish |
| Setup | magnetic field was generated with two shielded, 4-coil systems, respectively placed inside a µ-metal box chamber which was situated inside an incubator to maintain constant cell culture conditions; electric fields were below 1 V/m, no measurable temperature differences and vibrations (manufacturer information); coil current and chamber temperature were continuously monitored and temperature differences between exposed and sham exposed cells were below 0.1°C |
| Sham exposure | A sham exposure was conducted. |
| Additional info | for the sham exposure, the same setup was utilized but currents were switched non-parallel |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 2 mT | effective value | - | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 1 hour or 24 hours |
| Additional info | cell viability |
| Exposure source |
|
|---|---|
| Chamber | cells were cultured and exposed in a 96-well plate |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 2 mT | effective value | - | - | - |
No significant differences between exposed and sham exposed cells in any of the examined endpoints were found in both cell lines.
The authors conclude that the data demonstrated that 50 Hz magnetic fields did not have a genotoxic effect in embryonic mouse fibroblasts even in Atm-deficient cells.
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