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Medical/biological study (experimental study)

Ataxia telangiectasia mutated deficiency does not result in genetic susceptibility to 50 Hz magnetic fields exposure in mouse embryonic fibroblasts.

Published in: Bioelectromagnetics 2018; 39 (6): 476-484

Aim of study (acc. to author)

To examine the effects of 50 Hz magnetic fields on DNA damage in wild type embryonic mouse fibroblasts and in mutated (Atm-deficient) mouse embryonic fibroblasts.
Background/further details: In humans, ataxia telangiectasia (or Louis-Bar syndrome) is a rare autosomal recessive recessive disease caused by a mutation in the Atm-gene, which plays a central role in double-strand break repair. In research, Atm-deficient cells are used as a model to investigate genotoxic agents because they are extremely sensitive.
A positive control was conducted with 0.1 mM 4-nitroquinoline-1-oxide.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 15 minutes, 1 hour or 24 hours
genotoxicity tests and cell cycle distribution
Exposure 2: 50 Hz
Exposure duration: 1 hour or 24 hours
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 15 minutes, 1 hour or 24 hours
Additional info genotoxicity tests and cell cycle distribution
Exposure setup
Exposure source
Chamber cells were cultured and exposed in a dish
Setup magnetic field was generated with two shielded, 4-coil systems, respectively placed inside a µ-metal box chamber which was situated inside an incubator to maintain constant cell culture conditions; electric fields were below 1 V/m, no measurable temperature differences and vibrations (manufacturer information); coil current and chamber temperature were continuously monitored and temperature differences between exposed and sham exposed cells were below 0.1°C
Sham exposure A sham exposure was conducted.
Additional info for the sham exposure, the same setup was utilized but currents were switched non-parallel
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value - - -
Exposure 2
Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 1 hour or 24 hours
Additional info cell viability
Exposure setup
Exposure source
Chamber cells were cultured and exposed in a 96-well plate
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value - - -
Reference articles
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

No significant differences between exposed and sham exposed cells in any of the examined endpoints were found in both cell lines.
The authors conclude that the data demonstrated that 50 Hz magnetic fields did not have a genotoxic effect in embryonic mouse fibroblasts even in Atm-deficient cells.
Study character:

Study funded by

  • National Natural Science Foundation (NSFC), China
  • Ministry of Science and Technology (MOST), China

Related articles

  • Yoon YJ et al. (2015): Effects of 60-Hz Time-Varying Electric Fields on DNA Damage and Cell Viability Support Negligible Genotoxicity of the Electric Fields.
  • Yoon HE et al. (2014): Increased gamma-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines.
  • Nakayama M et al. (2014): DNA Strand Breaks in Fibroblasts Exposed to a 50-Hz Magnetic Field.
  • Kim J et al. (2012): Time-varying magnetic fields of 60 Hz at 7 mT induce DNA double-strand breaks and activate DNA damage checkpoints without apoptosis.
  • Kim J et al. (2010): Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells.
  • Focke F et al. (2010): DNA fragmentation in human fibroblasts under extremely low frequency electromagnetic field exposure.
  • Burdak-Rothkamm S et al. (2009): DNA and chromosomal damage in response to intermittent extremely low-frequency magnetic fields.
  • Winker R et al. (2005): Chromosomal damage in human diploid fibroblasts by intermittent exposure to extremely low-frequency electromagnetic fields.
  • Scarfi MR et al. (2005): Evaluation of genotoxic effects in human fibroblasts after intermittent exposure to 50 Hz electromagnetic fields: a confirmatory study.
  • Ivancsits S et al. (2003): Intermittent extremely low frequency electromagnetic fields cause DNA damage in a dose-dependent way.
  • Ivancsits S et al. (2002): Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts.