Study type: Medical/biological study (experimental study)

Ataxia telangiectasia mutated deficiency does not result in genetic susceptibility to 50 Hz magnetic fields exposure in mouse embryonic fibroblasts. med./bio.

Published in: Bioelectromagnetics 2018; 39 (6): 476-484

Aim of study (acc. to author)

To examine the effects of 50 Hz magnetic fields on DNA damage in wild type embryonic mouse fibroblasts and in mutated (Atm-deficient) mouse embryonic fibroblasts.

Background/further details

In humans, ataxia telangiectasia (or Louis-Bar syndrome) is a rare autosomal recessive recessive disease caused by a mutation in the Atm-gene, which plays a central role in double-strand break repair. In research, Atm-deficient cells are used as a model to investigate genotoxic agents because they are extremely sensitive.
A positive control was conducted with 0.1 mM 4-nitroquinoline-1-oxide.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 15 minutes, 1 hour or 24 hours
genotoxicity tests and cell cycle distribution
Exposure 2: 50 Hz
Exposure duration: 1 hour or 24 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 15 minutes, 1 hour or 24 hours
Additional info genotoxicity tests and cell cycle distribution
Exposure setup
Exposure source
Chamber cells were cultured and exposed in a dish
Setup magnetic field was generated with two shielded, 4-coil systems, respectively placed inside a µ-metal box chamber which was situated inside an incubator to maintain constant cell culture conditions; electric fields were below 1 V/m, no measurable temperature differences and vibrations (manufacturer information); coil current and chamber temperature were continuously monitored and temperature differences between exposed and sham exposed cells were below 0.1°C
Sham exposure A sham exposure was conducted.
Additional info for the sham exposure, the same setup was utilized but currents were switched non-parallel
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 1 hour or 24 hours
Additional info cell viability
Exposure setup
Exposure source
Chamber cells were cultured and exposed in a 96-well plate
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

No significant differences between exposed and sham exposed cells in any of the examined endpoints were found in both cell lines.
The authors conclude that the data demonstrated that 50 Hz magnetic fields did not have a genotoxic effect in embryonic mouse fibroblasts even in Atm-deficient cells.

Study character:

Study funded by

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