Study type: Medical/biological study (experimental study)

Increased gamma-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines. med./bio.

Published in: Int J Radiat Biol 2014; 90 (4): 291-298

Aim of study (acc. to author)

To examine whether extremely low frequency magnetic fieldsalone and in combination with ionizing radiation or hydrogen peroxide (H2O2) induce DNA damage in human lung fibroblasts and human lung epithelial cells.

Background/further details

Cells were subjected to different treatments: 1.) control group, 2.) magnetic field exposure alone (1 mT or 2 mT), 3.) magnetic field exposure (2 mT) in the presence of H2O2 (fibroblasts: 0.05mM; epithelial cells: 1 mM) and 4.) magnetic field exposure (2 mT) and afterwards radiation with gamma rays (1 Gy). As positive controls, the cells were treated with different doses of gamma rays (single dose 0-2 Gy, dose rate of 5 Gy/min) or cultivated with different concentrations of H2O2 (0-1 mM) for 6 hours.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 6 hours
Exposure 2: 60 Hz
Exposure duration: continuous for 6 hours
Exposure 3: 60 Hz
Exposure duration: continuous for 6 hours

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 6 hours
Additional info magnetic field alone
Exposure setup
Exposure source
  • not specified
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -
magnetic flux density 2 mT - - - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 6 hours
Additional info magnetic field + ionizing radiation
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Exposure 3

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 6 hours
Additional info magnetic field + hydrogen peroxide
Exposure setup
Chamber cells were cultivated and exposed in 60 mm cell culture dishes
Setup temperature in exposure chamber was 37°C ± 0.3°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Reference articles

  • Lee HJ et al. (2012): Combined effects of 60 Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In both cell lines, exposure to a magnetic field alone with the intensity of 2 mT led to a significant increase in DNA double strand breaks compared to the control group, while a magnetic field with the intensity of 1 mT had no significant effect. The combination of magnetic field exposure and gamma radiation led to significant more DNA double strand breaks in both cell lines than one of the treatments alone, while no synergistic effect was found regarding the combination of magnetic field exposure and H2O2.
The authors conclude that extremely low frequency magnetic fields could induce DNA damage in human lung fibroblasts and human lung epithelial cells. Additionally, the data indicate that magnetic field exposure and gamma radiation could act synergistically.

Study character:

Study funded by

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