Study type: Medical/biological study (experimental study)

Extremely low-frequency electromagnetic fields do not affect DNA damage and gene expression profiles of yeast and human lymphocytes. med./bio.

Published in: Radiat Res 2005; 164 (3): 277-285

Aim of study (acc. to author)

To study the effects of extremely low frequency electromagnetic fields on peripheral human blood lymphocytes and yeast.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 18 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 18 hours
Additional info reference article: D. Andreuccetti, M. Bini, A. Ignesti, R. Olmi, S. Priori and R. Vanni, Helmholtz coils for the generation of standard magnetic fields in biological applications. Proceedings of the VII National Congress of the AIFB, Monte Conero, Ancona (Italy), June 1992. Phys. Med. IX (Suppl. 1), 283-285 (1993).
Exposure setup
Exposure source
Setup The HC-50 exposure system consisted of a Helmholtz pair system comprising two parallel, coaxial, circular coils having a diameter of 40.6 cm. The distance between coils was equal to their radius (20.3 cm). Each coil was made up of 16 turns of 0.5-mm-thick enamelled copper wire. The two coils were connected in series so that the same current flowed in both. The field strength was regulated by varying the drive current supplied by an adjustable power unit allowing the HC-50 to produce a 50 Hz magnetic flux density varying from 1 to more than 150 µT.
Additional info Each lymphocyte population was divided into two aliquots. The exposed cell aliquots were incubated at the desired field intensity in a cell incubator containing the HC-50 Helmholtz coil system, and the unexposed cell aliquots were placed in a second incubator used in parallel. As an additional control, some experiments were performed by reversing the current in one of the coils in the HC-50 (sham exposure).
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 µT - measured - -
magnetic flux density 1 µT - calibration - -
magnetic flux density 10 µT - calibration - -
magnetic flux density 100 µT - calibration - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After exposure to the electromagnetic field, an increase in the amount of strand breaks or oxidated DNA bases relative to controls or a variation in gene expression profiles were not observed. The data suggest that extremely low frequency electromagnetic fields do not induce DNA damage or affect gene expression in these two different eukaryotic cell systems.

Study character:

Study funded by

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