|Exposure duration||continuous for 18 hours|
|Additional info||reference article: D. Andreuccetti, M. Bini, A. Ignesti, R. Olmi, S. Priori and R. Vanni, Helmholtz coils for the generation of standard magnetic fields in biological applications. Proceedings of the VII National Congress of the AIFB, Monte Conero, Ancona (Italy), June 1992. Phys. Med. IX (Suppl. 1), 283-285 (1993).|
|Setup||The HC-50 exposure system consisted of a Helmholtz pair system comprising two parallel, coaxial, circular coils having a diameter of 40.6 cm. The distance between coils was equal to their radius (20.3 cm). Each coil was made up of 16 turns of 0.5-mm-thick enamelled copper wire. The two coils were connected in series so that the same current flowed in both. The field strength was regulated by varying the drive current supplied by an adjustable power unit allowing the HC-50 to produce a 50 Hz magnetic flux density varying from 1 to more than 150 µT.|
|Additional info||Each lymphocyte population was divided into two aliquots. The exposed cell aliquots were incubated at the desired field intensity in a cell incubator containing the HC-50 Helmholtz coil system, and the unexposed cell aliquots were placed in a second incubator used in parallel. As an additional control, some experiments were performed by reversing the current in one of the coils in the HC-50 (sham exposure).|
After exposure to the electromagnetic field, an increase in the amount of strand breaks or oxidated DNA bases relative to controls or a variation in gene expression profiles were not observed. The data suggest that extremely low frequency electromagnetic fields do not induce DNA damage or affect gene expression in these two different eukaryotic cell systems.