Study type: Medical/biological study (experimental study)

Evaluation of the effects of extremely low frequency electromagnetic fields on mammalian follicle development. med./bio.

Published in: Hum Reprod 2000; 15 (11): 2319-2325

Aim of study (acc. to author)

The effects of exposure of mouse ovarian follicles to a 33 Hz or 50 Hz magnetic field on the follicle development should be investigated.

Background/further details

Pre-antral follicles were isolated from 22-day old mice and were either exposed to a 33 Hz magnetic field (group 1), a 50 Hz magnetic field (group 2) or not exposed (control group) for 5 days.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 33 Hz
Modulation type: pulsed
Exposure duration: continuous for 5 days
Exposure 2: 50 Hz
Modulation type: pulsed
Exposure duration: continuous for 5 days

Exposure 1

Main characteristics
Frequency 33 Hz
Type
Waveform
  • rectangular
Exposure duration continuous for 5 days
Additional info included harmonics: 99 Hz and 165 Hz
Modulation
Modulation type pulsed
Duty cycle 50 %
Exposure setup
Exposure source
Chamber plates with 96 wells (one follicle/well)
Setup field was homogeneous over 64 wells of a 96-well plate; plate was placed in central region of the coil (no contact); temperature was maintained at 37 ± 0.1°C inside each well, CO2 concentration was 5%
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.5 mT - measured - ± 0.01 mT

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
  • rectangular
Exposure duration continuous for 5 days
Additional info included harmonics: 150 Hz and 250 Hz
Modulation
Modulation type pulsed
Duty cycle 50 %
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.5 mT - measured - ± 0.01 mT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Growth was significantly reduced in 33 Hz-exposed follicles (group 1), but not in 50 Hz-exposed follicles (group 2), after 5 days compared to the control group. In both exposure groups, antrum formation was significantly impaired compared to the control group and follicles with failed antrum formation showed a significantly reduced concentration of estradiol in the culture medium (remark EMF-Portal: results for group 2 were not described distinctly). Cell proliferation was significantly reduced in comparison to the control group as well. Apoptosis was not significantly altered between the groups.
The authors conclude that exposure of mouse ovarian follicles to a 33 Hz or 50 Hz magnetic field might have detrimental effects on the follicle development.

Study character:

Study funded by

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