Study type: Medical/biological study (experimental study)

Ultrastructural alterations and occurrence of apoptosis in developing follicles exposed to low frequency electromagnetic field in rat ovary. med./bio.

Published in: Pak J Biol Sci 2007; 10 (24): 4413-4419

Aim of study (acc. to author)

To study electromagnetic field induced alterations of ovary follicles at the cellular level to find out whether electromagnetic field exposure can increase the incidence of apoptosis in follicular cells and oocytes.

Background/further details

30 female rats were divided into a control group and an exposure group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 4 h/day for 4 months

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 4 h/day for 4 months
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Compared to the control group, ultrastructural alterations in oocytes were present in the electromagnetic field exposed rats: Zona pellucida appeared condensed and narrower. Granulosa cells were condensed and lost contact with oocytes or neighbor-cells. The number of microvilli was decreased and in their cytoplasm several lipid droplets and organelles were dispersed. The nuclei of the oocytes appeared irregular in shape and the nucleoli were not as developed as in controls. Granulosa cells near the basal lamina showed features of apoptosis: nuclear condensation, changes in nuclear morphology, appearance of autophagic vacuoles and apoptotic bodies, separation of cells from neighboring cells.
Present results suggest that electromagnetic field exposure may interfere with normal processes of folliculogenesis.

Study character:

Study funded by

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