To investigate the effects of extremely low frequency magnetic fields on the oocyte differentiation and follicular development in mice.
Pregnant mice were divided into an exposure and a sham exposure group (n=15 per group). Exposure/sham exposure took place during the pregnancy. After delivery, half of the female pups of both groups were sacrificed, while the other half was kept under normal conditions (no exposure) and sacrificed when they reached adulthood.
Exposure duration: 4 hours/day during pregnancy (21 days)
|Exposure duration||4 hours/day during pregnancy (21 days)|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||3 mT||-||-||-||-|
Ovaries from exposed mice showed oocyte nests that were mostly broken and irregular arranged, while in the sham exposure group oocyte nests were intact and regularly arranged. Primordial follicles of mice from the exposure group were less developed than those from the sham exposure group and oocytes appeared to contain heterochromatin and were shrunken with vacuolated cytoplasm.
The authors conclude that extremely low frequency magnetic fields could influence the oocyte differentiation and follicular development in mice and that this may impair fertility.