Study type: Medical/biological study (experimental study)

Exposure to extremely low frequency magnetic fields suppresses x-ray-induced transformation in mouse C3H10T1/2 cells. med./bio.

Published in: Biochem Biophys Res Commun 2000; 271 (2): 323-327

Aim of study (acc. to author)

This study was undertaken to investigate the effects of extremely low frequency magnetic field on cell transformation of mouse cells in vitro.

Background/further details

Cell transformation was studied in X-rays exposed (at 3 Gy) and/or extremely low frequency magnetic field exposed cell cultures in medium plus/without TPA.
The experiments were repeated three times.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 24 h
Exposure 2: 60 Hz
Exposure duration: 6 weeks

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration 24 h
Additional info experiments were performed: i) with only EMF exposure ii) with X-rays and EMF exposure iii) with EMF exposure plus TPA iv) with X-rays, EMF exposure and TPA
Exposure setup
Exposure source
  • see Miyakoshi et. al. and Yaguchi et. al.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 mT maximum - - 5, 50, 400mT

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration 6 weeks
Additional info experiments were performed with: i) only EMF exposure ii) only X-ray irradiation iii) X-ray irradiation and EMF exposure iv) neither x-rays nor EMF exposure
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant difference in the cell transformation was found between sham-exposed and the extremely low frequency magnetic field exposed cells (in medium with/without TPA).
A lower cell transformation frequency was observed in co-exposed cells ( X-rays plus magnetic field) compared to X-rays alone. In medium containing TPA , the cell transformation frequency by X-rays alone was elevated more compared to co-exposed cells.
The long-term exposure at 5 mT suppressed both spontaneous and X-ray-induced cell transformations significantly.
Summarized the exposure to extremely low frequency magnetic field did not induce cell transformation, but suppressed the X-ray-induced cell transformation.

Study character:

Study funded by

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