Study type: Medical/biological study (experimental study)

Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 1999; 440 (2): 189-194

Aim of study (acc. to author)

To investigate the effects on the DNA and the cell survival of mouse cells exposed to extremely low frequency magnetic field in vitro.

Background/further details

The cells were exposed to extremely low frequency magnetic field alone, treated with mitomycin C (4, 10 and 40 nM) alone or treated with "mitomycin C plus extremely low frequency magnetic field exposure".

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 42 h
Exposure 2: 60 Hz
Exposure duration: continuous for 42 h
Exposure 3: 60 Hz
Exposure duration: continuous for 42 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 42 h
Exposure setup
Exposure source
Chamber Acrylic resin CO2 incubator with an inner space of 300 mm x 45 mm x 400 mm for exposure housing ten 10 cm culture dishes. For control experiments, a conventional incubator in a separate room was used.
Additional info The whole set-up was shielded by Permalloy C.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 50 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 42 h
Exposure setup
Exposure source
Chamber CO2 incubator with an exposure space of 14 cm height x 20 cm diameter housing sixteen 10 culture dishes.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT effective value measured - -

Exposure 3

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 42 h
Exposure setup
Exposure source
Chamber 600 silicon steel strips, each 0.5 mm thick making a core. Two copper coils (10 x 20 mm²) penetrate into the silicon steel core. The incubator (inner space 190 x 220 x 28 mm³) was inserted into the inner space of the core. Magnetic field was homogeneous within 1% difference for the area housing ten culture dishes.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 mT effective value measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The cells exposed to 400 mT extremely low frequency magnetic field exhibited a significant elevation of the sister chromatid exchange frequencies. No significant difference was observed in cells exposed to 5 and 50 mT extremely low frequency magnetic field.
The exposure to extremely low frequency magnetic field did not influence the proliferation index value.The proliferation index value in mitomycin C treated cells and in "mitomycin C treated cells plus extremely low frequency magnetic field exposure" were significantly lower.

Study character:

Study funded by

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