Study type: Medical/biological study (experimental study)

Kinetics of gene expression following exposure to 60 Hz, 2 mT magnetic fields in three human cell lines. med./bio.

Published in: Bioelectrochem Bioenerg 1997; 43 (1): 1-6

Aim of study (acc. to author)

To investigate in vitro effects of 60 Hz magnetic fields on gene expression in different cell lines.

Background/further details

c-myc, c-fos, ß-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Csa-19 gene expression were studied in human breast (MCF7 WT), brain (A 172) and cervix (HeLa S3) cells. The different p53 status of the cell lines was considered.
The positive control for gene expression of c-myc and c-fos in all cell lines was TPA. For Csa-19 gene expression 7 Gy gamma rays radiation was used to elicit a positive response.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 24 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 24 h
Exposure setup
Exposure source
Setup 25 cm² tissue culture flasks placed in the central planes of long solenoids. The cell monolayer was parallel to the field lines. The solenoids were enclosed in individual magnetic shield cylinders.
Sham exposure A sham exposure was conducted.
Additional info Control cells experienced zero magnetic field but were subjected to same level of disturbances such as acoustic noise and convective heating. 0.5 µg/ml TPA was administered for 30 min as positive control for expression of c-myc and c-fos in all cell lines. 7 Gy of X-rays was used to elicit a positive response in Csa-19.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT unspecified measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In all three cell lines there were no statistically significant magnetic field effects in c-myc, c-fos, ß-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Csa-19 gene expression.

Study character:

Study funded by

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