Study type: Medical/biological study (experimental study)

Extremely low-frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5 med./bio.

Published in: Environ Sci Pollut Res 2020; 27 (31): 39466-39473

Aim of study

The effects of exposure of human lung fibroblasts to a 50 Hz magnetic field on proliferation and oxidative homeostasis should be investigated.

Background/further details

Cells were divided into the following groups: 1) exposure for 1 day, 2) exposure for 2 days, 3) exposure for 3 days, 4) exposure for 7 days and 5) control group.
Cells were examined 24 hours after the end of exposure, respectively.



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 1 hour/day for 1, 2, 3 or 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Exposure duration 1 hour/day for 1, 2, 3 or 7 days
Exposure setup
Exposure source
Chamber cell culture flask and 96-well plates
Setup solenoid (60 cm diameter) placed in a incubator; cells were placed in the central area of the solenoid where the magnetic field was most uniform
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell proliferation was significantly increased after 2 and 7 days of exposure (groups 2 and 4) and significantly decreased after 3 days of exposure (group 3) compared to the control group. Several significant differences were found in parameters of oxidative homeostasis between exposure groups and the control group. The authors interpreted the results as follows: exposure to a magnetic field for more than 2 days exceeds the defense capacity of the cells antioxidative system, which in turn results in disruption of the cell cycle. This disruption is reflected by an increased cell proliferation induced by peroxynitrite accumulation (groups 2 and 4) and in the reduction of cell proliferation due to accumulation of cytotoxic oxidized glutathione (group 3).
The authors concluded that exposure of human lung fibroblasts to a 50 Hz magnetic field might affect proliferation and oxidative homeostasis depending on the exposure duration.

Study character:

Study funded by

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