Study type: Medical/biological study (experimental study)

The effect of 835.62 MHz FDMA or 847.74 MHz CDMA modulated radiofrequency radiation on the induction of micronuclei in C3H 10T(1/2) cells med./bio.

By: Bisht KS, Moros EG, Straube WL, Baty JD, Roti Roti JL
Published in: Radiat Res 2002; 157 (5): 506-515
Download citation in RIS format

Aim of study (acc. to author)

To determine if radiofrequency radiation induces the formation of micronuclei.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835.62 MHz
Exposure duration: continuous for 3, 8,16, or 24 h
Exposure 2: 847.74 MHz
Exposure duration: continuous for 3, 8,16, or 24 h

Exposure 1

Main characteristics
Frequency 835.62 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 3, 8,16, or 24 h
Additional info FDMA
Modulation
Modulation type cf. additional info
Additional info

FDMA
Exposure setup
Exposure source
Distance between exposed object and exposure source 29.2 cm
Chamber Each RTL was formed by two parallel metal plates, 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (1/4" thick); the TEM waves travelling radially from the central conical antenna (10.2 cm radius) were terminated by an annulus of MW-absorbing foam backed by a perforated aluminium lamina. The radius of the entire parallel region from the antenna's center to the lamina was 53.3 cm.
Setup Sixteen tightly capped T-75 flasks, each containing 40 ml of culture medium, were positioned in the region around the antenna at a mean radial position of 29.2 cm, for a total of 1200 cm² of culture area per RTL, and maintained at 37.0 ± 0.3°C.
Sham exposure A sham exposure was conducted.
Additional info The rather high SAR values were obtained by loading each flask dielectrically with a shim of alumina (Al2O3) ceramic placed under it.
Parameters
Measurand Value Type Method Mass Remarks
SAR 3.2 W/kg mean measured and calculated - for power setting 1
SAR 5.1 W/kg mean measured and calculated - for power setting 2
Additional parameter details

Monolayer cultures of cells were irradiated with 137Cs γ rays at doses of 0.1, 0.3, 0.6, 0.9, and 1.2 Gy and a dose rate of 0.816 Gy/min

Measurement and calculation details

Weighted mean SAR values over the flask bottom were measured using both thermometric and thermographic techniques and agreed reasonably with three-dimensional FDTD model calculations [Pickard et al., 2000].

Exposure 2

Main characteristics
Frequency 847.74 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 3, 8,16, or 24 h
Additional info CDMA
Modulation
Modulation type cf. additional info
Additional info

CDMA
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 3.2 W/kg mean measured and calculated - for power setting 1
SAR 4.8 W/kg mean measured and calculated - for power setting 2

Reference articles

  • Pickard WF et al. (2000): Experimental and numerical determination of SAR distributions within culture flasks in a dielectric loaded radial transmission line
  • Moros EG et al. (1999): The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks
  • Moros EG et al. (1998): A compact shielded exposure system for the simultaneous long-term UHF irradiation of forty small mammals: I. Electromagnetic and environmental design

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure of C3H10T1/2 cells in exponential growth or in plateauphase to 847.74 MHz CDMA or 835.62 MHz FDMA (at 3.2 W/kg for 24h) did not induce micronuclei.

Study character:

Study funded by

Related articles