Study type: Medical/biological study (experimental study)

900 MHz radiation does not induce micronucleus formation in different cell types. med./bio.

Published in: Mutagenesis 2012; 27 (4): 477-483

Aim of study (acc. to author)

To study the potential of radiofrequency electromagnetic fields (900 MHz) to cause a genomic damage in the form of micronucleus induction.

Background/further details

After different post-exposure incubation periods (4 h or 22 h, with or without cytochalasin B), cells were fixed and micronucleus frequencies were evaluated. Cells treated with methyl methanesulfonate for 4 h were used as positive control.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 30 min (both cell types) or 22 h (AL cells only)
both cell types
Exposure 2: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 30 min
AL cells only

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Charakteristic
Exposure duration continuous for 30 min (both cell types) or 22 h (AL cells only)
Additional info both cell types
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup cells placed in slide flasks positioned in the center of the µTEM cell; a µTEM cell is a widened coaxial waveguide in which the inner conductor transforms from a round wire at the input and output ports to a thin plate in the center; above and beneath this plate the field is homogeneous; slide flasks at the bottom of the housing underneath the plate; µTEM cell temperature-regulated with a Peltier element and placed inside a thermally isolating styrofoam box; µTEM cell terminated with a 50 Ω resistor to ensure travelling wave conditions
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 90 V/m effective value calculated - 5, 10, 30, 90 V/m

Exposure 2

Main characteristics
Frequency 900 MHz
Type
Charakteristic
Exposure duration continuous for 30 min
Additional info AL cells only
Modulation
Modulation type pulsed
Additional info

GSM - all eight time slots set to normal burst (GMSK/full rate)

Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 90 V/m effective value calculated - 5, 10, 30, 90 V/m

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Both cell types did not show any genomic damage after exposure. To adapt the protocol for the micronucleus test into the direction of the protocol for mitotic disturbances, the post-exposure incubation period was reduced (4 h). and exposure time was extended to one cell cycle length (22 h). This did not result in any increase of the genomic damage.
In conclusion, micronucleus induction was not observed as a consequence of exposure to non-ionizing radiation, even though this agent was reported to cause mitotic disturbances under similar experimental conditions (see Schmid and Schrader 2007, Schrader et al. 2008, Schrader et al. 2011).

Study character:

Study funded by

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