Study type: Medical/biological study (experimental study)

Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study. med./bio.

Published in: Radiat Res 2006; 165 (6): 655-663

Aim of study (acc. to author)

To study whether 24 h exposure to radiofrequency electromagnetic fields similar to those emitted by mobile phones induces genotoxic effects and/or effects on cell cycle kinetics in cultured human peripheral blood lymphocytes.

Background/further details

Genotoxic effects were evaluated in lymphocyte cultures from 10 healthy donors. Positive controls were provided by using mitomycin C (0.033 µg/ml).
Two research groups were involved in the study: one at ENEA, Rome, and the other at CNR-IREA, Naples. Each laboratory tested five donors, and the resulting slides were scored by both laboratories.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
  • SAR: 1 W/kg peak value
  • SAR: 5 W/kg peak value
  • SAR: 10 W/kg peak value

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 24 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Additional info

GSM signal

Exposure setup
Exposure source
  • wire-patch cell (WPC)
Chamber The exposure system consisted of four WPCs, each fed by a different power level in a blinded procedure. Two WPCs shielded by metal grid boxes were placed in each of two incubators (5% CO2 and 95% air). Each WPC or at least each incubator had a dummy culture Petri dish for temperature measurement.
Setup The WPC [Laval et al., 2000] consisted of two parallel plates (15 x 15 x 2.9 cm³), short-circuited at the edges by four posts. The outer conductor of the RF supply coaxial cable was connected to the top plate, and the inner conductor was connected to the ground plane. The temperature of the exposed samples was maintained at 36.9 ± 0.5°C by spiral coils with circulating water positioned on each plate. Four 35-mm Petri dishes containing 3 ml of diluted blood were placed inside the cell where the E-field distribution was quite uniform.
Sham exposure A sham exposure was conducted.
Additional info Six conditions were tested in duplicate: RF exposure at peak SARs of 0 (sham), 1, 5 and 10 W/kg, a positive (MMC-treated) control, and a negative (unexposed and untreated) control. Positive and negative controls were kept for 24 h in a third CO2 incubator at 37°C.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg peak value measured and calculated - -
SAR 5 W/kg peak value measured and calculated - -
SAR 10 W/kg peak value measured and calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data revealed no genotoxic or cytotoxic effects in the range of SAR values investigated. These findings were confirmed by two reserach groups and when the same slides were scored by two operators.

Study character:

Study funded by

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