Cellular DNA damage can be recognized and cell cycle stopped by so called "DNA damage cell cycle checkpoints". The examined cell line affords analysing G2 phase checkpoint. The G2 phase checkpoint stops cells entering mitosis with damaged chromosomes. Loss of checkpoint control is a feature of oncogenic transformation.
Cells were subjected to the following conditions at 37°C in a 5% CO2/air atmosphere: Group 1: control group, Group 2: no irradiation but exposed to MF, Group 3: irradiated with (137)CS at 1-5 Gy and no EMF exposure, Group 4: irradiated with (137)CS at 1-5 Gy and exposed to MF.
|Exposure duration||not specified in this article|
Cells exposed to extremely low frequency electromagnetic field alone had no effect on their progression into cell division compared to control group. Cells exposed to gamma radiation alone slowly regained their ability to enter and complete mitosis, but were considerably delayed compared to control group and electromagnetic field exposed group. Co-exposed cells (electromagnetic field and gamma radiation) similary showed a considerable delay in progressing through mitosis. However, co-exposed cells were entering G1 phase earlier than cells only exposed to gamma radiation, which would be consistent with a reduced G2 phase checkpoint control. This effect was more prominent with increasing doses of gamma radiation.