Study type: Medical/biological study (experimental study)

Possible attenuation of the G2 DNA damage cell cycle checkpoint in HeLa cells by extremely low frequency (ELF) electromagnetic fields. med./bio.

Published in: Cancer Cell Int 2002; 2 (1): 3

Aim of study (acc. to author)

To investigate the effects on cell cycle regulation in human cervical carcinoma cells exposed to extremely low frequency electromagnetic field and/or gamma radiation (1, 3, and 5 Gy) in vitro.

Background/further details

Cellular DNA damage can be recognized and cell cycle stopped by so called "DNA damage cell cycle checkpoints". The examined cell line affords analysing G2 phase checkpoint. The G2 phase checkpoint stops cells entering mitosis with damaged chromosomes. Loss of checkpoint control is a feature of oncogenic transformation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: not specified in this article

General information

Cells were subjected to the following conditions at 37°C in a 5% CO2/air atmosphere: Group 1: control group, Group 2: no irradiation but exposed to MF, Group 3: irradiated with (137)CS at 1-5 Gy and no EMF exposure, Group 4: irradiated with (137)CS at 1-5 Gy and exposed to MF.

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration not specified in this article
Exposure setup
Exposure source
Setup MF perpendicular to the cell monolayers.
Sham exposure A sham exposure was conducted.
Additional info The exposure system was housed in a 37°C hot room.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cells exposed to extremely low frequency electromagnetic field alone had no effect on their progression into cell division compared to control group. Cells exposed to gamma radiation alone slowly regained their ability to enter and complete mitosis, but were considerably delayed compared to control group and electromagnetic field exposed group. Co-exposed cells (electromagnetic field and gamma radiation) similary showed a considerable delay in progressing through mitosis. However, co-exposed cells were entering G1 phase earlier than cells only exposed to gamma radiation, which would be consistent with a reduced G2 phase checkpoint control. This effect was more prominent with increasing doses of gamma radiation.

Study character:

Study funded by

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