To determine potential immunomodulatory influences of pulse-modulated 1300 MHz microwave field on blood mononuclear cells (G0 phase cells) isolated from 16 healthy donors.
Modulation type: pulsed
Exposure duration: continuous for 1 h
|Exposure duration||continuous for 1 h|
|Additional info||vertically polarised|
|Pulse width||5 µs|
|Repetition frequency||330 Hz|
|Distance between exposed object and exposure source||1.5 m|
|Chamber||5.5 m long x 2.2 m wide x 2.1 m high|
|Setup||A specimen of 3 ml of isolated PBMC was placed in a sterile tube.|
|power density||6,000 W/m²||peak value||measured||-||-|
|power density||10 W/m²||mean||measured||-||-|
|electric field strength||61.4 V/m||mean||measured||-||-|
|SAR||0.18 W/kg||mean||determined by power loss||-||-|
The results indicate that the exposure of the cells influenced the monocyte-dependent immunoregulatory mechanisms responsible for the initiation of immune response. The exposure further significantly increased the production of IL-1 and decreased the production of IL-1ra. The changed ratio of IL-1/IL-1ra resulted in an increased value of lymphocyte-monocyte cooperation index, which reflects the activation of immunogenic and proinflammatory functions of monocytes. The proliferative response of T lymphocytes to mitogens (PHA, concanavalin A) was not changed. But the exposure significantly increased the production of IL-10, the immunoregulatory lymphokine that is involved in the T cell-monocyte interaction. The immunstimulatory effects of the 1300 MHz fields seem to affect preferentially the immunogenic functions of monocytes and the increased production of IL-10 seems to be a secondary reaction of T cells in response to activated monocytes.