Study type: Medical/biological study (experimental study)

Subchronic effects on leukocyte-endothelial interactions in mice by whole body exposure to extremely low frequency electromagnetic fields. med./bio.

Published in: In Vivo 2004; 18 (4): 425-432

Aim of study (acc. to author)

To measure and quantify changes in leukocyte behavior in the subcutaneous microcirculation in vivo and to examine serum cytokine levels in vitro while mice were exposed to continuous 50 Hz electromagnetic fields.

Background/further details

To visualize leukocytes in vivo the fluorescent marker rhodamine 6G was used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: repeated daily exposure, 20 h/day for 17 days
Exposure 2: 50 Hz
Exposure duration: repeated daily exposure, 20 h/day for 17 days
Exposure 3: 50 Hz
Exposure duration: repeated daily exposure, 20 h/day for 17 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration repeated daily exposure, 20 h/day for 17 days
Exposure setup
Exposure source
Setup A rack containing 18 cages was placed in the center of the coil system. The coil system (890 x 890 mm²) consisted of four spaced square coils which were crossed at right angles to each other and were regulated independently.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 300 µT effective value measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration repeated daily exposure, 20 h/day for 17 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration repeated daily exposure, 20 h/day for 17 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Significantly increased endothelial-adhering leukocytes were found only in the 3 mT exposure group. No changes were revealed in serum tumor necrosis factor alpha and interleukin 1 beta levels.
Subsequently, long-term continuous irradiation to 50 Hz electromagnetic fields elicited leukocyte-endothelium interactions, however, this effect does not appear to be controlled by cytokine levels.

Study character:

Study funded by

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