Study type: Medical/biological study (experimental study)

Evaluation of parameters of oxidative stress after in vitro exposure to FMCW- and CDMA-modulated radiofrequency radiation fields. med./bio.

Published in: Radiat Res 2004; 162 (5): 497-504

Aim of study (acc. to author)

To study whether radiofrequency irradiation emitted by cellular phones is capable of inducing oxidative stress or affecting the response to oxidative stress in cultured mammalian cells.

Background/further details

To evaluate the effect of radiofrequency exposure on oxidative stress, the cells were stimulated with interferon-gamma and bacterial lipopolysaccharide prior to exposure. In some experiments interferon-gamma-primed cells were supplemented of L-NIO (an inhibitor of nitric oxide synthase), at the time of stimulation with lipopolysaccharide to allow assessment of the effects of nitric oxide.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835.62 MHz
Modulation type: CW
Exposure duration: continuous for 20 to 22 h
  • SAR: 800 µW/g mean (± 0.13 µW/g)
Exposure 2: 847.74 MHz
Exposure duration: continuous for 20 to 22 h
  • SAR: 800 µW/g mean (± 0.13 µW/g)

Exposure 1

Main characteristics
Frequency 835.62 MHz
Type
Exposure duration continuous for 20 to 22 h
Modulation
Modulation type CW
Additional info

Frequency modulated continuous wave

Exposure setup
Exposure source
Setup Cells were exposed or sham exposed in T-75 flasks containing 40 ml of medium.
Parameters
Measurand Value Type Method Mass Remarks
SAR 800 µW/g mean cf. remarks - ± 0.13 µW/g

Exposure 2

Main characteristics
Frequency 847.74 MHz
Type
Exposure duration continuous for 20 to 22 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 800 µW/g mean cf. remarks - ± 0.13 µW/g

Reference articles

  • Moros EG et al. (1999): The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data of these studies indicate that FMCW (frequency-modulated continuous wave)- and CDMA-modulated radiofrequency irradiation did not alter parameters indicative of oxidative stress in J774.16 cells.
FMCW- and CDMA-modulated fields did not alter the level of intracellular oxidants, accumulation of glutathione disulfide or induction of antioxidant defenses in interferon-gamma/lipopolysaccharide-stimulated cells.
Consistent with the lack of an effect on oxidative stress parameters, no change in toxicity was revealed in the cells after either optimal (2 µg/ml of bacterial lipopolysaccharide) (with or without inhibitors of nitric oxide synthase) or suboptimal (0.5 µg/ml of bacetrial lipopolysaccharide) stimulation.

Study character:

Study funded by

Related articles