To evaluate the effect of radiofrequency exposure on oxidative stress, the cells were stimulated with interferon-gamma and bacterial lipopolysaccharide prior to exposure. In some experiments interferon-gamma-primed cells were supplemented of L-NIO (an inhibitor of nitric oxide synthase), at the time of stimulation with lipopolysaccharide to allow assessment of the effects of nitric oxide.
|Exposure duration||continuous for 20 to 22 h|
The data of these studies indicate that FMCW (frequency-modulated continuous wave)- and CDMA-modulated radiofrequency irradiation did not alter parameters indicative of oxidative stress in J774.16 cells.
FMCW- and CDMA-modulated fields did not alter the level of intracellular oxidants, accumulation of glutathione disulfide or induction of antioxidant defenses in interferon-gamma/lipopolysaccharide-stimulated cells.
Consistent with the lack of an effect on oxidative stress parameters, no change in toxicity was revealed in the cells after either optimal (2 µg/ml of bacterial lipopolysaccharide) (with or without inhibitors of nitric oxide synthase) or suboptimal (0.5 µg/ml of bacetrial lipopolysaccharide) stimulation.