Study type: Medical/biological study (experimental study)

Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields. med./bio.

Published in: Int J Radiat Biol 2006; 82 (8): 561-567

Aim of study (acc. to author)

To detect possible clastogenic and aneugenic properties of a 50 Hz magnetic field in newborn and adult mice.

Background/further details

Liver and peripheral blood samples (erythrocytes) from newborn mice exposed to an extremely low frequency magnetic field during the whole intrauterine life (21 days), and bone marrow and peripheral blood samples from adult mice exposed to the same magnetic field for the same period were investigated.
Micronuclei are produced after chromosome breakage (clastogenic damage, leads to a micronucleus with a chromosome fragment) or spindle disturbance (aneugenic damage, leads to micronucleus with a whole chromosome).
Positive control was carried out with X-rays (3 Gy).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 24 h/day for 21 days

General information

The experiment was performed with 4 groups: a) 15 adult mice b) 36 newborn mice from 4 pregnant mice which were exposed during pregnancy and three days after birth c) a control group of 15 adult mice which were not exposed d) a positiv control group with 6 adult mice which were exposed to X-rays

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 24 h/day for 21 days
Exposure setup
Exposure source
Chamber the solenoid was 0.8 m in length and 0.13 m in radius, with 552 turns of 2.5 mm² copper wire. The solenoid was not shielded.
Additional info A control group of another 15 mice was kept un-exposed for 21 days. Positive control was carried out exposing 6 adult mice to X-rays.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 650 µT effective value unspecified - at the centre of the solenoid

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Data of newborn mice showed a significant increase in micronuclei frequencies. In absolute terms, most of the induced micronuclei were CREST-negative (i.e. formed by a chromosome fragment). However, in relative terms, extremely low frequency exposure caused a two-fold increase in CREST-negative micronuclei and a four-fold increase in CREST-positive micronuclei (i.e. formed by a whole chromosome).
No significant effect was recorded on exposed adult mice.
In conclusion, further studies are needed to investigate the possible link between electromagnetic fields and aneuploidy, because the importance of aneuploidy in relation to carcinogenesis.

Study character:

Study funded by

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