Study type: Medical/biological study (experimental study)

Effects of static and 50 Hz alternating electric fields on superoxide dismutase activity and TBARS levels in guinea pigs. med./bio.

Published in: Gen Physiol Biophys 2006; 25 (2): 177-193

Aim of study (acc. to author)

This in vivo study was performed to determine the effects of static and 50 Hz alternating electric fields on lipid peroxidation and superoxide dismutase levels (antioxidant) in different organ tissues of guinea pigs.

Background/further details

Investigations were focused on the influence of different electric field intensities, electric field directions and exposure duration.
140 male guinea pigs were exposed to static electric fields, divided into 14 separate groups of 10 animals each. The control group consisted of 20 guinea pigs. Additionally, 5 groups of 15 male guinea pigs were exposed to 50 Hz.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: 8 h/day for 3 days
vertical field
Exposure 2:
Exposure duration: 8 h/day für 3 days
horizontal field
Exposure 3: 50 Hz
Exposure duration: 8 h/day for 1, 3, 5, 7 or 10 days
vertical AC field

Exposure 1

Main characteristics
Frequency
Type
Exposure duration 8 h/day for 3 days
Additional info vertical field
Exposure setup
Exposure source
Chamber Animals were placed in wooden cages. Copper plates (50 x 50 x 0.1 cm) spaced 14 cm, were mounted on the top and bottom faces of the cages (50 x 50 x 14 cm) to form parallel plates of a capacitor. Control animals were kept under the same conditions without electric field.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 0.3 kV/m minimum measured - 0.3, 0.6, 0.8, 1, 1.35, 1.5, 1.8 KV/m

Exposure 2

Main characteristics
Frequency
Type
Exposure duration 8 h/day für 3 days
Additional info horizontal field
Exposure setup
Exposure source
Chamber same as in E1 but the plates were mounted on the left and right side of the cage.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 0.3 kV/m minimum measured - 0.3, 0.6, 0.8, 1, 1.35, 1.5, 1.8 KV/m

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 h/day for 1, 3, 5, 7 or 10 days
Additional info vertical AC field
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 1.35 kV/m - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Statistically significant differences were found in the thiobarbituric acid reactive substances and superoxide dismutase contents of plasma and tissues between the exposed groups (static electric field (both vertical and horizontal electric field direction) and 50 Hz alternating electric field ) and control group.
Increase in the electric field intensity or electric field exposure duration resulted in an increase in the thiobarbituric acid reactive substance levels and superoxide dismutase activity detected in plasma and all tissues.
The data obtained indicate that electric field in the range of several kV/m is effective on thiobarbituric acid reactive substance levels and superoxide dismutase activity.

Study character:

Study funded by

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