Study type: Medical/biological study (experimental study)

Electromagnetic fields used clinically to improve bone healing also impact lymphocyte proliferation in vitro. med./bio.

Published in: Biomed Sci Instrum 2001; 37: 215-220

Aim of study (acc. to author)

This study was performed to examine the T-cell response of humans and rats to sinusoidal or pulsed electromagnetic field exposure.

Background/further details

Peripheral, predominantly mature T-cells were obtained from blood of healthy human volunteers and rats. Rat T-cells were additionally isolated from spleen to investigate the proliferative capacity of immature lymphocytes. The exposure groups was performed with/without anti-CD3 (T-cell receptor activation) or concanavalin A.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 20 min
Exposure 2: 60 Hz
Modulation type: pulsed
Exposure duration: 4 h/day for 21 days
Exposure 3:
  • unspecified
Exposure duration: not given in the article
Exposure 4: 100 Hz
Exposure duration: not given in the article

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 20 min
Additional info cell cultures
Exposure setup
Exposure source
Setup double wound, 38 cm square Merrit coils, cell cultures placed in the center of the coil system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 4 h/day for 21 days
Additional info rats
Modulation
Modulation type pulsed
Exposure setup
Exposure source
  • commercial wound-healing PEMF using a coil system and signal generator
Setup rats placed in the center of the coils system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.2 mT effective value measured - -

Exposure 3

Main characteristics
Frequency
  • unspecified
Type
Waveform
Exposure duration not given in the article
Additional info cell cultures
Exposure setup
Exposure source
Setup culture cells placed in the center of the coil system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.8 mT - measured - -

Exposure 4

Main characteristics
Frequency 100 Hz
Type
Waveform
Exposure duration not given in the article
Additional info cell cultures
Exposure setup
Exposure source
Setup culture cells placed in the center of the coil system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.2 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to sinusoidal and pulsed electromagnetic field of human and rat peripheral blood T-cells showed an increased cell proliferation with/without anti-CD3 or concanavalin A compared to control group.
In vivo investigations on rats exposed to the pulsed electromagnetic field revealed increased splenic T-cell proliferation (anti-CD3 activated) and depressed peripheral T-cell proliferation.

Study character:

Study funded by

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