Study type: Medical/biological study (experimental study)

Myocardial function improved by electromagnetic field induction of stress protein hsp70. med./bio.

Published in: J Cell Physiol 2008; 216 (3): 816-823

Aim of study (acc. to author)

To examine whether pre-treatment with electromagnetic-field induced levels of stress protein hsp70 can preserve myocardial function after ischemia and re-perfusion.

Background/further details

Studies on myocardial function have shown that hsp70, stimulated by an increase in temperature, leads to improved survival following ischemia and re-perfusion. Male rats were randomly assigned to a control group (n=10) and to an exposure group (n=10), that was irradiated to electromagnetic fields for 30 min prior to ischemia and re-perfusion. Ischemia was induced by ligation of left anterior descending coronary artery for 30 min, followed by 30 min re-perfusion.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 30 min
Exposure setup
Exposure source
Setup 16 cm x 24 cm plastic exposure cage surrounded by Helmholtz coils; coils consisting of 164 turns of 1.5 inches thick 19 gauge copper wire covered with electrical tape
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 µT - measured - -

Reference articles

  • Goodman R et al. (1998): Magnetic field stress induces expression of hsp70.
  • Jin M et al. (1997): Biological and technical variables in myc expression in HL60 cells exposed to 60 Hz electromagnetic fields.
  • Wei LX et al. (1990): Changes in levels of c-myc and histone H2B following exposure of cells to low-frequency sinusoidal electromagnetic fields: evidence for a window effect.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Significant upregulation of the HSP70 gene and increased hsp70 levels were measured in response to electromagnetic field pre-exposure. Hemodynamics demonstrated significant recovery of systolic contractile function after 30 min of re-perfusion following electromagnetic field exposure. Ventricular diastolic function was markedly improved in electromagnetic field-treated animals.
Non-invasive electromagnetic field induction of hsp70 preserved myocardial function and has the potential to improve tolerance to ischemic injury.

Study character:

Study funded by

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