Study type: Medical/biological study (experimental study)

Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes. med./bio.

Published in: Biol Open 2019; 8 (8): bio041293

Aim of study (acc. to author)

The possible genotoxic effects of a 50 Hz magnetic field exposure in cardiomyocytes should be investigated in vitro and in vivo.

Background/further details

For the in vitro experiments, human cardiomyocytes were divided into the following groups: 1) continuous exposure to the magnetic field, 2) intermittent exposure to the magnetic field as well as a control group and a sham exposure group. Moreover, for cell cycle analyses and comet assay, positive controls were used. Experiments were repeated three times.
For the in vivo experiments, 20 rats were randomly and evenly divided into an exposure (group 3) and a sham exposure group. All animals were sacrificed the day after the last exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 1 hour
Exposure 2: 50 Hz
Exposure duration: intermittent (15 min field on / 15 min field off) for 75 min
Exposure 3: 50 Hz
Exposure duration: continuous for 15 hours/day for 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Additional info in vitro
Exposure setup
Exposure source
Setup a pair of parallel coils (200 cm × 70 cm × 200 cm, L × W × H; horizontal bronze wires were used to generate a vertical magnetic field; in order to generate the uniform magnetic field, the wiring ratio of the five coils was 2:1:1:1:2 and the 3% uniformity area for each floor was 150 cm × 50 cm (L × W); the wires were fixed tightly to the framework in order to decrease the vibration and the noise; temperature during exposure was 22°C
Sham exposure A sham exposure was conducted.
Additional info sham-exposure system was exactly the same system as the exposure system but without power; it was located in a separate and non-adjacent room with the same environmental conditions
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration intermittent (15 min field on / 15 min field off) for 75 min
Additional info in vitro
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 15 hours/day for 7 days
Additional info in vivo
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Reference articles

  • Zhou L et al. (2016): The effects of a 50-Hz magnetic field on the cardiovascular system in rats.
  • Lai J et al. (2016): Effects of 100 µT extremely low frequency electromagnetic fields exposure on hematograms and blood chemistry in rats.
  • Misakian M et al. (1993): Biological, physical, and electrical parameters for in vitro studies with ELF magnetic and electric fields: a primer.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

There were no statistically significant effects in any exposure group compared to the control groups or sham exposure groups.
The authors conclude that there were no indications of genotoxic effects of 50 Hz magnetic field exposure in cardiomyocytes in vitro and in vivo found.

Study character:

Study funded by

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