To study the effects of radiofrequency electromagnetic fields on connexin gene expression and protein expression, as well as connexin localization and ultrastructural cell features in gestational tissue (trophoblast cell line).
|Repetition frequency||217 Hz|
|SAR||2 W/kg||average over time||measured||-||-|
Radiofrequency exposure significantly and selectively increased gene expression of connexin 40 and connexin 43, without altering protein expression. Nevertheless, connexin 40 and connexin 43 lost their punctate fluorescence within the cell membrane (compared to sham exposed cells), becoming diffuse after exposure. Thus, localization of connexin 40 and connexin 43 was significantly changed following exposure. Electron microscopy evidenced a sharp decrease in intercellular gap junction-like structures. Cell viability and cell functionality were not significantly influenced by electromagnetic field exposure.
The data indicate that exposure of extravillous trophoblast to GSM-217 Hz signals can modify connexin gene expression, connexin protein localization and cellular ultrastructure.