研究のタイプ: 医学/生物学の研究 (experimental study)

[栄養膜のコネキシンに対する高周波電磁界の影響] med./bio.

Effect of high-frequency electromagnetic fields on trophoblastic connexins.

掲載誌: Reprod Toxicol 2009; 28 (1): 59-65

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研究目的(著者による)

To study the effects of radiofrequency electromagnetic fields on connexin gene expression and protein expression, as well as connexin localization and ultrastructural cell features in gestational tissue (trophoblast cell line).

詳細情報

Connexins are membrane proteins that are able to influence the trophoblast functions.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,817 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h
  • SAR: 2 W/kg average over time

ばく露1

主たる特性
周波数 1,817 MHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two 128.5 mm x 65 mm x 424 mm brass single mode waveguide resonators; petri dishes kept on plastic holders inside the resonators at the H-field maximum of the standing wave
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg average over time 測定値 - -

Reference articles

  • Valbonesi P et al. (2008): [1.8 GHz振幅変調無線周波電磁界にばく露したヒト絨毛細胞株におけるHSP70発現とDNA損傷の評価]
  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Radiofrequency exposure significantly and selectively increased gene expression of connexin 40 and connexin 43, without altering protein expression. Nevertheless, connexin 40 and connexin 43 lost their punctate fluorescence within the cell membrane (compared to sham exposed cells), becoming diffuse after exposure. Thus, localization of connexin 40 and connexin 43 was significantly changed following exposure. Electron microscopy evidenced a sharp decrease in intercellular gap junction-like structures. Cell viability and cell functionality were not significantly influenced by electromagnetic field exposure.
The data indicate that exposure of extravillous trophoblast to GSM-217 Hz signals can modify connexin gene expression, connexin protein localization and cellular ultrastructure.

研究の種別:

研究助成

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