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To study the effects of radiofrequency electromagnetic fields on connexin gene expression and protein expression, as well as connexin localization and ultrastructural cell features in gestational tissue (trophoblast cell line).
Connexins are membrane proteins that are able to influence the trophoblast functions.
Modulation type: pulsed
ばく露時間: continuous for 1 h
|ばく露時間||continuous for 1 h|
|Repetition frequency||217 Hz|
|ばく露装置の詳細||two 128.5 mm x 65 mm x 424 mm brass single mode waveguide resonators; petri dishes kept on plastic holders inside the resonators at the H-field maximum of the standing wave|
|Sham exposure||A sham exposure was conducted.|
|SAR||2 W/kg||average over time||測定値||-||-|
Radiofrequency exposure significantly and selectively increased gene expression of connexin 40 and connexin 43, without altering protein expression. Nevertheless, connexin 40 and connexin 43 lost their punctate fluorescence within the cell membrane (compared to sham exposed cells), becoming diffuse after exposure. Thus, localization of connexin 40 and connexin 43 was significantly changed following exposure. Electron microscopy evidenced a sharp decrease in intercellular gap junction-like structures. Cell viability and cell functionality were not significantly influenced by electromagnetic field exposure.
The data indicate that exposure of extravillous trophoblast to GSM-217 Hz signals can modify connexin gene expression, connexin protein localization and cellular ultrastructure.