Study type: Medical/biological study (experimental study)

Comparison of Hsps expression after radio-frequency field exposure in three human glioma cell lines. med./bio.

Published in: Biomed Environ Sci 2009; 22 (5): 374-380

Aim of study (acc. to author)

To study and compare the effects of radiofrequency electromagnetic field exposure on expression of heat shock proteins in three human glioma cell lines.

Background/further details

Cells treated at 43°C for 3 h served as a positive control.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,950 MHz
Modulation type: CW
Exposure duration: continuous for 1 h

Exposure 1

Main characteristics
Frequency 1,950 MHz
Type
Exposure duration continuous for 1 h
Additional info corresponding to the middle frequency of the IMT 2000 uplink
Modulation
Modulation type CW
Exposure setup
Exposure source
  • not specified
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 W/kg - - - -
SAR 10 W/kg - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Hsp27 was primarily located within the cytoplasm, p-Hsp27 in both cytoplasm and nuclei of the three cell lines. Radiofrequency electromagnetic field exposure did not affect the distribution or expression of Hsp27. After radiofrequency electromagnetic field exposure (10 W/kg, 1 h), the p-Hsp27 immunofluorescence became slightly weaker in M054 cells than in A172 and T98 cells compared to sham exposed cells.
In addition, the data showed no significant differences in protein expression of Hsp27 or Hsp70 between sham exposed and exposed cell lines. There was also no difference in phosphorylated Hsp27 level between the three cell lines exposed to radiofrequency fields at a SAR value of 1 W/kg and sham exposed ceIIs. However, exposure to radiofrequency electromagnetic field at a SAR of 10 W/kg for 1 h slightly decreased the protein level of phosphorylated Hsp27 in MO54 cells.
The authors conclude, that 1950 MHz radiofrequency exposure has only little or no apparent effect on Hsp70 and Hsp27 expression in MO54, A172, and T98 cells.

Study character:

Study funded by

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