Study type: Medical/biological study (experimental study)

900-MHz microwave radiation enhances gamma-ray adverse effects on SHG44 cells. med./bio.

Published in: J Toxicol Environ Health A 2009; 72 (11): 727-732

Aim of study (acc. to author)

To study whether pre-microwave exposure might enhance gamma radiation-induced damage in human glioma cells.

Background/further details

Cells were divided into four groups: 1) control group, 2) ionizing radiation (60Co, 5 Gy) group, 3) microwave exposure group, and 4) "microwave + ionizing radiation" exposure group. Group 3 and 4 were further divided into different subgroups according to the intensity of microwave exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Exposure duration: continuous for 2 h/day on 3 days

General information

at day four after EMF exposure part of the cells were exposed to Co γ-rays at a dose of 5 Gy with a dose rate of 1Gy/min

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 2 h/day on 3 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 2 mW/cm² minimum - - -
power density 4 mW/cm² - - - -
power density 6 mW/cm² maximum - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure of glioma cells to 900 MHz (group 3; 4 mW/cm² and 6 mW/cm² ) microwaves and exposure to 6 mW/cm² in combination with gamma radiation resulted in a significant inhibition of cell proliferation. Furthermore, pre-exposure with 900 MHz microwaves followed by gamma radiation significantly increased the rate of apoptosis in a dose-dependent manner.
Both, 900 MHz microwave exposure and gamma radiation did not produce significant effects on cell cycle phase distribution.
The co-exposure of electromagnetic fields and gamma radiation resulted in a synergistic effect by triggering stress response, which increased reactive oxygen species (increase of MDA, decrease of superoxide dismutase in group 4 (with 6 mW/cm²)), but the expression of HSP70 remained unchanged.
The data indicate that the adverse effects of gamma radiation on cellular functions are strengthened by electromagnetic fields.

Study character:

Study funded by

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