研究のタイプ: 医学/生物学の研究 (experimental study)

[3種類のヒト神経膠腫細胞株での無線周波電磁界ばく露後のHsps発現の比較] med./bio.

Comparison of Hsps expression after radio-frequency field exposure in three human glioma cell lines.

掲載誌: Biomed Environ Sci 2009; 22 (5): 374-380

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研究目的(著者による)

To study and compare the effects of radiofrequency electromagnetic field exposure on expression of heat shock proteins in three human glioma cell lines.

詳細情報

Cells treated at 43°C for 3 h served as a positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,950 MHz
Modulation type: CW
ばく露時間: continuous for 1 h

ばく露1

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h
Additional information corresponding to the middle frequency of the IMT 2000 uplink
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • 詳細不明
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg - - - -
SAR 10 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Hsp27 was primarily located within the cytoplasm, p-Hsp27 in both cytoplasm and nuclei of the three cell lines. Radiofrequency electromagnetic field exposure did not affect the distribution or expression of Hsp27. After radiofrequency electromagnetic field exposure (10 W/kg, 1 h), the p-Hsp27 immunofluorescence became slightly weaker in M054 cells than in A172 and T98 cells compared to sham exposed cells.
In addition, the data showed no significant differences in protein expression of Hsp27 or Hsp70 between sham exposed and exposed cell lines. There was also no difference in phosphorylated Hsp27 level between the three cell lines exposed to radiofrequency fields at a SAR value of 1 W/kg and sham exposed ceIIs. However, exposure to radiofrequency electromagnetic field at a SAR of 10 W/kg for 1 h slightly decreased the protein level of phosphorylated Hsp27 in MO54 cells.
The authors conclude, that 1950 MHz radiofrequency exposure has only little or no apparent effect on Hsp70 and Hsp27 expression in MO54, A172, and T98 cells.

研究の種別:

研究助成

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