Study type: Medical/biological study (experimental study)

Effect of coexposure to 50 Hz magnetic fields and an aneugen on human lymphocytes, determined by the cytokinesis block micronucleus assay. med./bio.

Published in: Bioelectromagnetics 2003; 24 (3): 160-164

Aim of study (acc. to author)

To investigate the effects of the co-exposure of 50 Hz extremely low frequency magnetic field plus the aneugen vinblastine on micronucleus formation in human lymphocyte in vitro.

Background/further details

The lymphocytes cultures were separated from blood sample of 18 subjects.
Vinblastine was applied in four different concentrations: 5, 10, and 15 ng/ml.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 72 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 72 h
Exposure setup
Exposure source
Setup cylindrical coil 42 cm long with an inner diameter of 20 cm and 380 turns
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 80 µT - measured - -
magnetic flux density 800 µT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Increased vinblastine concentrations resulted in an increased micronucleus and apoptosis frequency and a decreased nuclear division index.
No statistically significant effect of extremely low frequency magnetic field alone on micronucleus frequency or apoptosis was observed, but the nuclear division index was significantly higher in the 800 µT group compared to control group, suggesting an effect of extremely low frequency magnetic field on cell proliferation.
Co-exposed cells revealed no statistically significant effect on micronucleus induction or apoptosis frequency, but a significant decrease in nuclear division index compared to only extremely low frequency magnetic field exposed cells.

Study character:

Study funded by

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