Study type: Medical/biological study (experimental study)

Investigation of the effects of 2.1 GHz microwave radiation on mitochondrial membrane potential (DeltaPsim), apoptotic activity and cell viability in human breast fibroblast cells. med./bio.

Published in: Cell Biochem Biophys 2013; 67 (3): 1371-1378

Aim of study (acc. to author)

To examine the effects of exposure to a Wideband Code Division Multiple Access signal (W-CDMA) on cell viability and apoptosis in human breast fibroblasts.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.1 GHz
Exposure duration: continuous for 4 or 24 hours

Exposure 1

Main characteristics
Frequency 2.1 GHz
Type
Exposure duration continuous for 4 or 24 hours
Exposure setup
Exposure source
Chamber cells were grown in 24-well culture plates in humidified cell incubator at 37°C under 5 % CO2 and 95 % air
Setup 24 well-plate was placed above the transmitting antenna
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 0.142 mW/cm² - - - -
SAR 0.607 W/kg spatial average calculated 10 g -
power 1 W - - - input power

Reference articles

  • Zeni O et al. (2012): Radiofrequency radiation at 1950 MHz (UMTS) does not affect key cellular endpoints in neuron-like PC12 cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the exposed cell cultures, the cell viability was significantly decreased compared to the corresponding sham exposure. Annexin V and propidium iodide staining showed a significantly increased apoptosis rate in exposed cell cultures in comparison to the sham exposed cell cultures. However, no significant changes between exposed and sham exposed cell cultures occurred regarding the level of tumor necrosis factor receptor. The mitochondrial membrane potential was significantly decreased in exposed cell cultures compared to sham exposure.
The data indicate that exposure of human breast fibroblasts to a W-CDMA signal could reduce the cell viability and induce apoptosis via a mitochondrial pathway.

Study character:

Study funded by

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