The TEM cell (107 x 51 x 51 cm, septum height 23 cm) was positioned vertically inside a thermostatic room maintained at 37°C.
Microplates were located in the middle section of the cell on the right and on the left side of the septum on a thin plastic shelf (2 mm).
A 96 well microplate (127 x 84 x 15 mm) or two 24 well microplates (127 x 84 x 22 mm) placed one upon the other were used. Well diameters and volume of culture medium were 6.4 or 16 mm and 200 µl or 2 ml, respectively.
The TEM cell reflection and transmission coefficients were measured using a network analyser, and the transmission coefficient was used to determine the cell input power. The signal at the amplifier output was measured with a peak power meter. The field inside the microplate was computed by the FDTD technique. Microplates were irradiated by a plane wave with the E-field (1 V/m) tangential to the bottom. Peak and averaged values of the field in the cell layer were computed, and the field dispersion around the average value was calculated by statistical analysis. Most of the cells were irradiated at the averaged E-field level with a small dispersion.
Sannino A et al.
Adaptive response in human blood lymphocytes exposed to non-ionizing radiofrequency fields: resistance to ionizing radiation-induced damage.
Esmekaya MA et al.
Investigation of the effects of 2.1 GHz microwave radiation on mitochondrial membrane potential (DeltaPsim), apoptotic activity and cell viability in human breast fibroblast cells.
Zeni O et al.
Induction of an adaptive response in human blood lymphocytes exposed to radiofrequency fields: Influence of the universal mobile telecommunication system (UMTS) signal and the specific absorption rate.
Palumbo R et al.
Exposure to 900 MHz radiofrequency radiation induces caspase 3 activation in proliferating human lymphocytes.
Hoyto A et al.
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Sanchez S et al.
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Chauhan V et al.
Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines.