Study type: Medical/biological study (experimental study)

ELF-EMFs induced effects on cell lines: controlling ELF generation in laboratory. med./bio.

Published in: Progr Electromagn Res B (PIER B) 2010; 24: 131-153

Aim of study (acc. to author)

The aim of the study was twofold: 1) to investigate the production of reactive oxygen species and mitochondrial membrane potential of different cell models (neuronal, glial and skeletal muscle cells) during in vitro cell exposure to extremely low frequency electromagnetic fields and 2) to describe the protocols, in particular the equipment developed to guarantee a controlled exposure of the biological models at a 50 Hz electromagnetic field.
Additionally, data of previous studies on animal models (Falone et al. 2008) and cellular models with prokaryotic cells (Cellini et al. 2008) were also summarized. Details of those studies are not given in the present extract.



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 30 min (acute exposure) or 7 days (chronic exposure)

Exposure 1

Main characteristics
Frequency 50 Hz
Exposure duration continuous for 30 min (acute exposure) or 7 days (chronic exposure)
Exposure setup
Exposure source
Setup Helmholtz coils with a side length of 828 mm, a separation of 451 mm and 64 turns of AWG (American Wire Gauge) 12 wire
Sham exposure A sham exposure was conducted.
Additional info whether the chronic exposure was also done with Helmholtz coils or instead with a solenoid is not stated clearly in the article
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT minimum - - -
magnetic flux density 1 mT maximum - - -

Reference articles

  • Di Loreto S et al. (2009): Fifty hertz extremely low-frequency magnetic field exposure elicits redox and trophic response in rat-cortical neurons.
  • Cellini L et al. (2008): Bacterial response to the exposure of 50 Hz electromagnetic fields.
  • Falone S et al. (2008): Chronic exposure to 50Hz magnetic fields causes a significant weakening of antioxidant defence systems in aged rat brain.
  • Falone S et al. (2007): Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells.
  • Simko M et al. (2004): Extremely low frequency electromagnetic fields as effectors of cellular responses in vitro: possible immune cell activation.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The data indicated that the cell response (ROS production and/or mitochondrial membrane potential) after acute exposure to extremely low frequency electromagnetic fields strictly depended on the cell model rather than on the applied magnetic field strength or duration of exposure. In both neuronal-like (PC12) and glial (GL15)-like cell lines a significant increase of reactive oxygen species production was detected, with a different time-course in each cell line. In GL15 cells, this probably caused the decrease of mitochondrial membrane potential also observed in C2C12 cells.
Cell proliferation or cell differentiation did not change after chronic exposure to extremely low frequency electromagnetic fields up to 1 mT.

Study character:

Related articles