The aim of the study was twofold: 1) to investigate the production of reactive oxygen species and mitochondrial membrane potential of different cell models (neuronal, glial and skeletal muscle cells) during in vitro cell exposure to extremely low frequency electromagnetic fields and 2) to describe the protocols, in particular the equipment developed to guarantee a controlled exposure of the biological models at a 50 Hz electromagnetic field.
Additionally, data of previous studies on animal models (Falone et al. 2008) and cellular models with prokaryotic cells (Cellini et al. 2008) were also summarized. Details of those studies are not given in the present extract.
|Setup||Helmholtz coils with a side length of 828 mm, a separation of 451 mm and 64 turns of AWG (American Wire Gauge) 12 wire|
|Sham exposure||A sham exposure was conducted.|
|Additional info||whether the chronic exposure was also done with Helmholtz coils or instead with a solenoid is not stated clearly in the article|
The data indicated that the cell response (ROS production and/or mitochondrial membrane potential) after acute exposure to extremely low frequency electromagnetic fields strictly depended on the cell model rather than on the applied magnetic field strength or duration of exposure. In both neuronal-like (PC12) and glial (GL15)-like cell lines a significant increase of reactive oxygen species production was detected, with a different time-course in each cell line. In GL15 cells, this probably caused the decrease of mitochondrial membrane potential also observed in C2C12 cells.
Cell proliferation or cell differentiation did not change after chronic exposure to extremely low frequency electromagnetic fields up to 1 mT.