Study type: Medical/biological study (experimental study)

The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells. med./bio.

Published in: J Radiat Res 2017; 58 (4): 474-486

Aim of study (acc. to author)

The effects of exposure of different neurogenic cell lines to a 50 Hz magnetic field on cell functions and DNA damage should be investigated to explore the possible association between extremely low frequency magnetic field exposure and the risk of nervous system diseases.

Background/further details

Neurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured neurogenic cells from rats (astrocytes, microglia, cortical neurons) were examined. All cells were exposed to the magnetic field for 1 hour, 6 hours and 24 hours. Cortical neurons were additionally exposed for 1 hour/day for 13 days. For each exposure group, a separate sham exposure group was used. Furthermore, positive controls were used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 1, 6 and 24 hours
all cells
Exposure 2: 50 Hz
Exposure duration: intermittent 1 hour/day for 13 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 1, 6 and 24 hours
Additional info all cells
Exposure setup
Exposure source
Setup two identical chambers (one for exposure, the other one for sham exposure) containing a series of Helmholtz coils were placed inside a cell culture incubator (37°C, 5% CO2) temperature variance between the chambers did not exceed 0.1°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent 1 hour/day for 13 days
Additional info only cortical neurons
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

No significant differences were found between exposed and sham-exposed cells.
The authors conclude that the present study does not indicate any effects of exposure of different neurogenic cell lines to a 50 Hz magnetic field on cell functions and DNA damage.

Study character:

Study funded by

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