The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells
Published in: J Radiat Res 2017; 58 (4): 474-486
Aim of study (acc. to author)
- cell viability/cell division/proliferation: cell viability (directly, 24 h and 48 h after exposure; spectrophotometry), cell proliferation (directly, 24 h and 48 h after exposure; cell count with hemocytometer), cell cycle distribution (directly, 6 h and 24 h after exposure; propidium iodide stain, flow cytometry); only cortical neurons: neuron morphology (immunofluorescence stain of tau protein (after 2 days exposure; marker for axons), MAP2 (after 6 days exposure; marker for dendrites), PSD95 and gephyrin (after 13 days exposure; markers for excitatory and inhibitory synapses, respectively); fluorescence microscopy)
- genotoxicity/mutation: DNA double strand breaks (directly after exposure; immunofluorescence stain of gamma-H2AX foci, fluorescence microscopy)
- cell function: only astrocytes and microglia: cytokine release (directly after exposure; levels of TNF-α, IL-6 and IL-1β in culture medium, ELISA), only microglia: phagocytosis (fluorescent microspheres uptake, fluorescence microscopy)
Time of investigation:
- during exposure
- after exposure
Main outcome of study (acc. to author)
Study funded by
National Natural Science Foundation (NSFC), China
Ministry of Science and Technology (MOST), China
Ministry of Environmental Protection (MEP), China
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