The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells
Su L, Yimaer A, Wei X, Xu Z, Chen G
Published in: J Radiat Res 2017; 58 (4): 474-486
Aim of study (acc. to author)
- cell viability/cell division/proliferation: cell viability (directly, 24 h and 48 h after exposure; spectrophotometry), cell proliferation (directly, 24 h and 48 h after exposure; cell count with hemocytometer), cell cycle distribution (directly, 6 h and 24 h after exposure; propidium iodide stain, flow cytometry); only cortical neurons: neuron morphology (immunofluorescence stain of tau protein (after 2 days exposure; marker for axons), MAP2 (after 6 days exposure; marker for dendrites), PSD95 and gephyrin (after 13 days exposure; markers for excitatory and inhibitory synapses, respectively); fluorescence microscopy)
- genotoxicity/mutation: DNA double strand breaks (directly after exposure; immunofluorescence stain of gamma-H2AX foci, fluorescence microscopy)
- cell function: only astrocytes and microglia: cytokine release (directly after exposure; levels of TNF-α, IL-6 and IL-1β in culture medium, ELISA), only microglia: phagocytosis (fluorescent microspheres uptake, fluorescence microscopy)
Time of investigation:
- during exposure
- after exposure
Main outcome of study (acc. to author)
Study funded by
National Natural Science Foundation (NSFC), China
Ministry of Science and Technology (MOST), China
Ministry of Environmental Protection (MEP), China
Lv Y et al.
Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology
Naarala J et al.
Direction-Dependent Effects of Combined Static and ELF Magnetic Fields on Cell Proliferation and Superoxide Radical Production
Reale M et al.
Effect of environmental extremely low-frequency electromagnetic fields exposure on inflammatory mediators and serotonin metabolism in a human neuroblastoma cell line
Ma Q et al.
Extremely low-frequency electromagnetic fields promote in vitro neuronal differentiation and neurite outgrowth of embryonic neural stem cells via up-regulating TRPC1
Duong CN et al.
Exposure to electromagnetic field attenuates oxygen-glucose deprivation-induced microglial cell death by reducing intracellular Ca(2+) and ROS
de Groot MW et al.
In vitro developmental neurotoxicity following chronic exposure to 50 Hz extremely low frequency electromagnetic fields (ELF-EMF) in primary rat cortical cultures
Benassi B et al.
Extremely low frequency magnetic field (ELF-MF) exposure sensitizes SH-SY5Y cells to the pro-Parkinson's disease toxin MPP+
Mun GI et al.
Effects of 60-Hz magnetic fields on DNA damage responses in HT22 mouse hippocampal cell lines
Cheng Y et al.
Extremely low-frequency electromagnetic fields enhance the proliferation and differentiation of neural progenitor cells cultured from ischemic brains
Ma Q et al.
Extremely low-frequency electromagnetic fields affect transcript levels of neuronal differentiation-related genes in embryonic neural stem cells
Seong Y et al.
Egr1 mediated the neuronal differentiation induced by extremely low-frequency electromagnetic fields
Trillo MA et al.
Retinoic acid inhibits the cytoproliferative response to weak 50Hz magnetic fields in neuroblastoma cells
Sulpizio M et al.
Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells
Luukkonen J et al.
Pre-Exposure to 50 Hz Magnetic Fields Modifies Menadione-Induced Genotoxic Effects in Human SH-SY5Y Neuroblastoma Cells
Marcantonio P et al.
Synergic effect of retinoic acid and extremely low frequency magnetic field exposure on human neuroblastoma cell line BE(2)C
Saito A et al.
Developmental effects of low frequency magnetic fields on P19-derived neuronal cells
Piacentini R et al.
Extremely low-frequency electromagnetic fields promote in vitro neurogenesis via upregulation of Ca(v)1-channel activity
Koyama S et al.
Extremely low frequency (ELF) magnetic fields enhance chemically induced formation of apurinic/apyrimidinic (AP) sites in A172 cells
Pozzi D et al.
Effect of 50 Hz magnetic field exposure on neuroblastoma morphology
Zhao TY et al.
Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes
Sul AR et al.
Effects of sinusoidal electromagnetic field on structure and function of different kinds of cell lines
Bodega G et al.
Acute and chronic effects of exposure to a 1-mT magnetic field on the cytoskeleton, stress proteins, and proliferation of astroglial cells in culture
Pirozzoli MC et al.
Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line
Wei M et al.
Exposure to 60 Hz magnetic fields and proliferation of human astrocytoma cells in vitro