Study type: Medical/biological study (experimental study)

Nonpulsed sinusoidal electromagnetic fields as a noninvasive strategy in bone repair: the effect on human mesenchymal stem cell osteogenic differentiation. med./bio.

Published in: Tissue Eng Part C Methods 2015; 21 (2): 207-217

Aim of study (acc. to author)

The effects of exposure of human mesenchymal stem cells to a combined static and 50 Hz magnetic field on osteogenesis and differentiation towards a bone phenotype should be investigated.

Background/further details

The combined static and 50 Hz magnetic field was used to generate a Ca2+-ion cyclotron resonance frequency, which was found to have biological effects in former studies by the authors (Ledda et al. (2013), De Carlo et al. (2012), Gaetani et al. (2009)).
Dexamethasone, a glucocorticoid, was used as a positive control and in a co-exposure, as it induces the osteogenic differentiation of human mesenchymal stem cells.
Cells were divided into the following groups: 1) combined magnetic field, 2) dexamethasone (100 nM), 3) combined magnetic field and dexamethasone and 4) untreated control group.
Cells were exposed after 3, 4 and 5 weeks of cultivation for 3 and 5 days, respectively, and were analyzed thereafter. Additionally, cells were examined 3 days after termination of exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0–50 Hz
Exposure duration: continuous for 3 or 5 days

Exposure 1

Main characteristics
Frequency 0–50 Hz
Type
Waveform
Exposure duration continuous for 3 or 5 days
Exposure setup
Exposure source
Chamber mu-metal shielded room
Setup exposure system consisted of a cellular incubator made of polymethyl methacrylate with 37°C ± 0.1°C and 5% CO2 and a solenoid (5-mm-thick polyvinyl chloride cylinder with 3.3 m length and 33 cm diameter made of 3300 turns of 1 mm diameter copper wire)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 66 µT - - - static magnetic field
magnetic flux density 1 mT effective value - - 50 Hz magnetic field

Reference articles

  • Ledda M et al. (2013): Non ionising radiation as a non chemical strategy in regenerative medicine: Ca(2+)-ICR "In Vitro" effect on neuronal differentiation and tumorigenicity modulation in NT2 cells.
  • De Carlo F et al. (2012): Non- ionizing radiation as a non invasive strategy in regenerative medicine: the effect of Ca2+-ICR on Mouse Skeletal Muscle Cell growth and differentiation.
  • Gaetani R et al. (2009): Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields.
  • Liboff AR (1985): Geomagnetic cyclotron resonance in living cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell proliferation was significantly reduced in the magnetic field exposure group (group 1) and MF/dexamethasone co-exposure group (group 3) compared to the control group and dexamethasone exposure group (group 2), respectively, in 3- and 4-week-old cell cultures. After 5 weeks, however, proliferation was significantly increased in group 1 compared to the control group.
Morphologically, groups 1 and 2 showed distinct differences which had larger, polygonal-shaped cells and a widespread actin network compared to the control group with elongated spindle-shaped cells and peripheral actin filaments. In group 3, this appearance was even more pronounced.
The gene expressions of several osteogenic markers, except for osteoprotegerin, were significantly upregulated in group 1 (MF alone) and group 3 (co-exposure) compared to the control group and group 2 (dexamethasone alone), respectively (remark EMF-Portal: results in text and figure are contradictory and not clear). The gene expression of osteoprotegerin was significantly reduced in groups (co-)exposed to dexamethasone (2 and 3) and significantly increased in group 2 compared to the control group.
The protein expressions of alkaline phosphatase and osteopontin were significantly upregulated in all exposure groups 1-3 compared to the control group.
The authors conclude that exposure of human mesenchymal stem cells to a combined static and 50 Hz magnetic field could induce a differentiation towards a bone phenotype and a sustainable stimulation of osteogenesis, what might be of therapeutic use.

Study character:

Study funded by

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