研究のタイプ: 医学/生物学の研究 (experimental study)

[非侵襲的な骨修復の手段としての非パルス化正弦波電磁界:ヒト間葉系幹細胞の骨細胞への分化に対する影響] med./bio.

Nonpulsed sinusoidal electromagnetic fields as a noninvasive strategy in bone repair: the effect on human mesenchymal stem cell osteogenic differentiation.

掲載誌: Tissue Eng Part C Methods 2015; 21 (2): 207-217

この研究は、ヒト間葉幹細胞(hMSC)の骨細胞への分化に対する50Hz(著者らはカルシウムイオンサイクロトロン共鳴を仮定してこの周波数を選択したと述べている)の非パルス正弦波電磁界ばく露単独の影響およびデキサメタゾンとの相乗的影響を調べた。その結果、5日間の電磁界連続ばく露が、hMSCの増殖、形態、細胞骨格アクチン再構成に影響した;さらに、骨分化マーカであるRunx2発現なども増加した;オステオポンチンおよびアルカリホスファターゼタンパク質発現も増加した、と報告している。

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研究目的(著者による)

The effects of exposure of human mesenchymal stem cells to a combined static and 50 Hz magnetic field on osteogenesis and differentiation towards a bone phenotype should be investigated.

詳細情報

The combined static and 50 Hz magnetic field was used to generate a Ca2+-ion cyclotron resonance frequency, which was found to have biological effects in former studies by the authors (Ledda et al. (2013), De Carlo et al. (2012), Gaetani et al. (2009)).
Dexamethasone, a glucocorticoid, was used as a positive control and in a co-exposure, as it induces the osteogenic differentiation of human mesenchymal stem cells.
Cells were divided into the following groups: 1) combined magnetic field, 2) dexamethasone (100 nM), 3) combined magnetic field and dexamethasone and 4) untreated control group.
Cells were exposed after 3, 4 and 5 weeks of cultivation for 3 and 5 days, respectively, and were analyzed thereafter. Additionally, cells were examined 3 days after termination of exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 0–50 Hz
ばく露時間: continuous for 3 or 5 days

ばく露1

主たる特性
周波数 0–50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 3 or 5 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 mu-metal shielded room
ばく露装置の詳細 exposure system consisted of a cellular incubator made of polymethyl methacrylate with 37°C ± 0.1°C and 5% CO2 and a solenoid (5-mm-thick polyvinyl chloride cylinder with 3.3 m length and 33 cm diameter made of 3300 turns of 1 mm diameter copper wire)
パラメータ
測定量 種別 Method Mass 備考
磁束密度 66 µT - - - static magnetic field
磁束密度 1 mT effective value - - 50 Hz magnetic field

Reference articles

  • Ledda M et al. (2013): [再生医療における非化学的戦略としての非電離放射線:NT2細胞のニューロン分化および腫瘍形成能修飾への“イン・ビトロ”でのCa(2+)-ICRへの影響]
  • De Carlo F et al. (2012): [再生医療における非侵襲的戦略としての非電離放射線:マウス骨格筋細胞の増殖と分化に対するCa2 + -ICRの影響]
  • Gaetani R et al. (2009): [超低周波電磁界にばく露されたヒト成人の心筋幹細胞の細胞分化]
  • Liboff AR (1985): [生きた細胞における地磁気サイクロトロン共鳴]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Cell proliferation was significantly reduced in the magnetic field exposure group (group 1) and MF/dexamethasone co-exposure group (group 3) compared to the control group and dexamethasone exposure group (group 2), respectively, in 3- and 4-week-old cell cultures. After 5 weeks, however, proliferation was significantly increased in group 1 compared to the control group.
Morphologically, groups 1 and 2 showed distinct differences which had larger, polygonal-shaped cells and a widespread actin network compared to the control group with elongated spindle-shaped cells and peripheral actin filaments. In group 3, this appearance was even more pronounced.
The gene expressions of several osteogenic markers, except for osteoprotegerin, were significantly upregulated in group 1 (MF alone) and group 3 (co-exposure) compared to the control group and group 2 (dexamethasone alone), respectively (remark EMF-Portal: results in text and figure are contradictory and not clear). The gene expression of osteoprotegerin was significantly reduced in groups (co-)exposed to dexamethasone (2 and 3) and significantly increased in group 2 compared to the control group.
The protein expressions of alkaline phosphatase and osteopontin were significantly upregulated in all exposure groups 1-3 compared to the control group.
The authors conclude that exposure of human mesenchymal stem cells to a combined static and 50 Hz magnetic field could induce a differentiation towards a bone phenotype and a sustainable stimulation of osteogenesis, what might be of therapeutic use.

研究の種別:

研究助成

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