研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト神経芽細胞腫細胞株に対するELF-EMFばく露の影響:プロテオミクス解析] med./bio.

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis.

掲載誌: Iran J Cancer Prev 2014; 7 (1): 22-27

この研究は、ヒト神経芽細胞腫細胞株(SH-SY5Y)にELF-EMFばく露(50 Hz磁界、2mT、3時間ばく露)を与え、タンパク質発現の変化をプロテオ−ム解析した。その結果、ばく露群では対照群に比べ、189種類のタンパク質発現に変化が見られた;主成分分析によりこれらのタンパク質データは実験条件によるクラスターに分けられた、と報告している。

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研究目的(著者による)

The potential effects of an in vitro exposure of human neuroblastoma cells to a 50 Hz magnetic field on the protein expression should be investigated.

詳細情報

For protein expression analysis, each sample (protein extracts from control and exposed cells) was loaded on three gels and investigated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 3 h

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 3 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 Helmholtz coils installed inside an incubator.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Morphological differences were spotted between exposed cells and control during microscopic analysis (no details described). Protein expression analysis showed a significant up-regulation or down-regulation of 189 proteins in the samples of exposed cells compared to control.
The authors conclude that an in vitro exposure of human neuroblastoma cells to a 50 Hz magnetic field has effects on the protein expression which in turn affects cell morphology. However, more studies are necessary to identify the proteins affected and to clarify further relationships.

研究の種別:

研究助成

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