Study type: Medical/biological study (experimental study)

BIGEL analysis of gene expression in HL60 cells exposed to X rays or 60 Hz magnetic fields. med./bio.

Published in: Radiat Res 1998; 150 (6): 663-672

Aim of study (acc. to author)

To discover genes that are differentially expressed in HL60 cells exposed to 60 Hz magnetic fields or X rays radiation (2 Gy/min as positive control) compared to unexposed cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 3 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 3 h
Additional info The generated magnetic field was linearly polarized and was nearly pure 60 Hz with a third harmonic component 20 to 40 decibels lower than the fundamental.
Exposure setup
Exposure source
Setup solenoid (38.7 cm long, 12 cm in diameter, 199 turns, 1.59 mH); tissue culture flasks placed horizontally in the central planes of long solenoids wound on thin-walled Plexiglas cylinders enclosed in magnetic shield cylinders, all of them were placed parallel to each other in water bath; water bath insulated with Styrofoam (1.2 m long, 0.7 m wide, 11 cm deep); solenoid used for sham control was bifilar wound; each solenoid was shielded by mu-metal enclosures
Additional info X-ray irradiation was performed at a dose rate of 2 Gy/min. Control cells were placed in the X-ray room for the same period.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT unspecified measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In approximately 1% of random selected cDNAs (in total 1920) a different gene expression was observed by X rays radiation (i.e. in 18 clones) but not by 60 Hz magnetic fields.

Study character:

Study funded by

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