Study type: Medical/biological study (experimental study)

Effect of Pulsed Electromagnetic Field on Bone Formation and Lipid Metabolism of Glucocorticoid-Induced Osteoporosis Rats through Canonical Wnt Signaling Pathway. med./bio.

Published in: Evid Based Complement Alternat Med 2016: 4927035-

Aim of study (acc. to author)

The effects of exposure to a pulsed 50 Hz magnetic field on bone formation and lipid metabolism and the underlying signal pathways should be investigated in a rat osteoporosis model.

Background/further details

Glucocorticoid-induced osteoporosis should be investigated. Therefore, all rats, except for the control group (group 4, see below), were injected with dexamethasone sodium phosphate injection (2.5 mg/kg body weight) into their right haunch muscles twice a week, 12 weeks prior to (sham) exposure to the pulsed magnetic field.
Rats were divided into 4 groups (n=10 each): 1) exposure to the pulsed magnetic field, 2) sham exposure and daily oral calcium supplement (56.25 mg/kg body weight), 3) only sham exposure, 4) sham exposed control group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Modulation type: pulsed
Exposure duration: 40 minutes/day for 12 weeks

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
  • rectangular
Exposure duration 40 minutes/day for 12 weeks
Modulation
Modulation type pulsed
Pulse width 200 µs
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 4 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

After exposure to the pulsed magnetic field for 12 weeks (group 1), the whole body bone mineral density was significantly increased, serum lipid levels were significantly decreased and trabecular bone (osseus tissue) in vertebra was significantly thicker compared to sham exposed GIOP rats (group 3).
The gene expressions and/or protein expressions of Wnt10b, LRP5, beta-catenin, OPG and Runx2 were significantly upregulated and Axin2, RANKL, PPAR-gamma, C/EBP-alpha, FABP4 and Dkk-1 were significantly downregulated in group 1 compared to group 3.
The authors conclude that exposure to a pulsed 50 Hz magnetic field might prevent bone loss and improve lipid metabolism disorders in osteoporosis model rats via the Wnt signaling pathway.

Study character:

Study funded by

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