研究のタイプ: 医学/生物学の研究 (experimental study)

[グルココルチコイド誘発性骨粗鬆症ラットの骨形成および脂質代謝に対するパルス化電磁界の古典的Wnt信号伝達経路を介した影響] med./bio.

Effect of Pulsed Electromagnetic Field on Bone Formation and Lipid Metabolism of Glucocorticoid-Induced Osteoporosis Rats through Canonical Wnt Signaling Pathway.

掲載誌: Evid Based Complement Alternat Med 2016: 4927035-

この研究は、グルココルチコイド誘発性骨粗鬆症(GIOP)ラットを用いて、その骨形成および脂質代謝に対するパルス電磁界PEMF)の影響、さらにはGIOPラットにおける古典的Wnt信号伝達経路の重要な構成要素および標的を探索した。その結果、PEMFばく露終了から12週間後において、全身の骨密度(BMD)レベルが有意に上昇し、血清脂質レベルが有意に低下し、骨梁厚が増加した;このようなPEMFばく露の影響に古典的Wnt信号伝達経路が重要な役割を果たすことが示された、と報告している。

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研究目的(著者による)

The effects of exposure to a pulsed 50 Hz magnetic field on bone formation and lipid metabolism and the underlying signal pathways should be investigated in a rat osteoporosis model.

詳細情報

Glucocorticoid-induced osteoporosis should be investigated. Therefore, all rats, except for the control group (group 4, see below), were injected with dexamethasone sodium phosphate injection (2.5 mg/kg body weight) into their right haunch muscles twice a week, 12 weeks prior to (sham) exposure to the pulsed magnetic field.
Rats were divided into 4 groups (n=10 each): 1) exposure to the pulsed magnetic field, 2) sham exposure and daily oral calcium supplement (56.25 mg/kg body weight), 3) only sham exposure, 4) sham exposed control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
Modulation type: pulsed
ばく露時間: 40 minutes/day for 12 weeks

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 40 minutes/day for 12 weeks
Modulation
Modulation type pulsed
Pulse width 200 µs
ばく露装置
ばく露の発生源/構造
  • ZH-21 osteoporosis treatment system
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 4 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After exposure to the pulsed magnetic field for 12 weeks (group 1), the whole body bone mineral density was significantly increased, serum lipid levels were significantly decreased and trabecular bone (osseus tissue) in vertebra was significantly thicker compared to sham exposed GIOP rats (group 3).
The gene expressions and/or protein expressions of Wnt10b, LRP5, beta-catenin, OPG and Runx2 were significantly upregulated and Axin2, RANKL, PPAR-gamma, C/EBP-alpha, FABP4 and Dkk-1 were significantly downregulated in group 1 compared to group 3.
The authors conclude that exposure to a pulsed 50 Hz magnetic field might prevent bone loss and improve lipid metabolism disorders in osteoporosis model rats via the Wnt signaling pathway.

研究の種別:

研究助成

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