研究のタイプ: 医学/生物学の研究 (experimental study)

[多能性幹細胞に超低周波電磁界が誘導した成長と骨形成分化の増強] med./bio.

Enhanced growth and osteogenic differentiation of Induced Pluripotent Stem cells by Extremely Low-Frequency Electromagnetic Field.

掲載誌: Cell Mol Biol (Noisy-le-grand) 2015; 61 (1): 36-41

この研究は、培養した人工多能性幹細胞(iPS細胞)の骨形成分化能に対する、長期間のパルス超低周波電磁界(50Hz、1.5mT)の影響力を調べた。電磁界放射下でのiPS細胞の生物学的挙動をMTT試験で評価した後、アルカリ性リン酸分解酵素(ALP)活性、カルシウムミネラル沈着等の重要な骨形成マーカー、ならびに重要な骨関連遺伝子を介して、幹細胞骨形成分化を調べた。MTT試験の結果、ELF電磁界での刺激後、iPS細胞の増殖率が有意に上昇した。ELF電磁界骨形成培地の両方の下で培養した細胞では、ELF電磁界または骨形成培地への単独ばく露の下で培養した場合と比較して、幹細胞骨形成の潜在能力が有意に上昇した。著者らはこれらの結果から、ELF電磁界骨形成培地の組合せは、幹細胞の骨分化を強く促進するので、骨欠損および骨粗鬆症患者の治療に利用できる、と結論付けている。

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研究目的(著者による)

The effects of exposure of induced pluripotent stem cells to a pulsed 50 Hz magnetic field on osteogenic differentiation should be investigated.

詳細情報

Cells were divided into 4 groups: 1) exposure to the magnetic field, 2) treatment with osteogenic medium (3 mM beta-glycerophosphate, 50 µg/ml ascorbic acid and 10-9 M dexamethasone), 3) co-exposure to the magnetic field and osteogenic medium, 4) control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
Modulation type: pulsed
ばく露時間: for 8 hours/day for 1, 3, 5, 7 or 14 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 for 8 hours/day for 1, 3, 5, 7 or 14 days
Modulation
Modulation type pulsed
Pulse width 350 ns
ばく露装置
ばく露の発生源/構造
  • ELF generator
ばく露装置の詳細 6 well culture plates were placed in exposure apparatus inside a CO2 incubator (5% CO2, 37°C)
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

After 5 and 7 days, all exposure groups (groups 1-3) showed a significantly increased proliferation compared to the control group. However, the co-exposure group (group 3) showed the highest value, respectively, which was even significantly higher than in groups 1 and 2.
Mineralization of the extracellular matrix was most distinct in group 3 as well and the calcium content, enzyme activity of alkaline phosphatase and the gene expression of most osteogenesis-related genes (collagen type 1, osteocalcin, alkaline phosphatase, osteoprotegerin, matrix metalloproteinases 1 and 3) were also most distinct in group 3 and significantly increased after 7 and/or 14 days compared to groups 1 and 2.
The authors conclude that exposure to a pulsed 50 Hz magnetic field in combination with osteogenic medium might be beneficial for osteogenic differentiation of induced pluripotent stem cells. These findings may be of therapeutical use.

研究の種別:

研究助成

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