The cells were divided into the following groups: 1) exposure to a 0.5 mT magnetic field for 20 minutes/day, 2) exposure to a 0.5 mT magnetic field for 40 minutes/day, 3) exposure to a 1 mT magnetic field for 20 minutes/day, 4) exposure to a 1 mT magnetic field for 40 minutes/day, 5) sham exposure for 20 minutes/day, 6) sham exposure for 40 minutes/day.
All experiments were repeated three times.
|Setup||solenoid was made of 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field at the center of the solenoid, where the cell cultures were located, was ±1%; the temperature in solenoid during exposure was controlled by a digital thermometer and magnetic field-induced heating was negligible|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.5 mT||-||measured||-||-|
|magnetic flux density||1 mT||-||measured||-||-|
In the trypan blue dye exclusion test, cell viability and cell proliferation were significantly increased in all exposure groups (groups 1-4) compared to sham exposure groups (groups 5 and 6). In the MTT test, cell viability and cell proliferation were significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the sham exposure groups. No differences in cell morphology were found between the exposure and sham exposure groups.
The authors conclude that exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field might promote cell proliferation.