Study type: Medical/biological study (experimental study)

Effect of extremely low-frequency (50 Hz) field on proliferation rate of human adipose-derived mesenchymal stem cells. med./bio.

Published in: J Radiobiol 2014; 1 (2): 31-37

Aim of study (acc. to author)

The effects of exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field on cell proliferation should be investigated.

Background/further details

The cells were divided into the following groups: 1) exposure to a 0.5 mT magnetic field for 20 minutes/day, 2) exposure to a 0.5 mT magnetic field for 40 minutes/day, 3) exposure to a 1 mT magnetic field for 20 minutes/day, 4) exposure to a 1 mT magnetic field for 40 minutes/day, 5) sham exposure for 20 minutes/day, 6) sham exposure for 40 minutes/day.
All experiments were repeated three times.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 20 to 40 minutes/day for 7 days
Exposure 2: 50 Hz
Exposure duration: 20 to 40 minutes/day for 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 20 to 40 minutes/day for 7 days
Exposure setup
Exposure source
Chamber 96-well plates
Setup solenoid was made of 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field at the center of the solenoid, where the cell cultures were located, was ±1%; the temperature in solenoid during exposure was controlled by a digital thermometer and magnetic field-induced heating was negligible
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 20 to 40 minutes/day for 7 days
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the trypan blue dye exclusion test, cell viability and cell proliferation were significantly increased in all exposure groups (groups 1-4) compared to sham exposure groups (groups 5 and 6). In the MTT test, cell viability and cell proliferation were significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the sham exposure groups. No differences in cell morphology were found between the exposure and sham exposure groups.
The authors conclude that exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field might promote cell proliferation.

Study character:

Study funded by

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