Study type: Medical/biological study (experimental study)

The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells. med./bio.

Published in: Acta Odontol Scand 2020: 1-7 [in press]

Aim of study (acc. to author)

The effects of exposure of dental pulp stem cells to 50 Hz magnetic fields on cell viability and genotoxicity should be investigated.

Background/further details

The study background was a possible use in regenerative endodontic procedures.
Cells were divided into the following groups: exposure to a magnetic field of 1) 0.5 mT for 20 min/day, 2) 0.5 mT for 40 min/day, 3) 1 mT for 20 min/day, 4) 1 mT for 40 min/day, 5) control group for 20 min/day exposure, 6) control group fpr 40 min/day exposure. All cells were investigated after 7 days of exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 20 min/day or 40 min/day for 7 days
Exposure 2: 50 Hz
Exposure duration: 20 min/day or 40 min/day for 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Waveform
Exposure duration 20 min/day or 40 min/day for 7 days
Exposure setup
Exposure source
Setup The solenoid was wound with 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field was ± 1% at the centre of the solenoid where the cell cultures were located
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - calibration - -

Exposure 2

Main characteristics
Frequency 50 Hz
Waveform
Exposure duration 20 min/day or 40 min/day for 7 days
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - calibration - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In all exposure groups (groups 1-4), cells extended on the surfaces of the culture vessels as the fibroblastic cells in contrast to the control groups. Cell viability was significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the control groups, with group 3 (1 mT for 20 min/day) showing the largest increase. Genotoxicity was at least signficantly increased in groups 2 (0,5 mT for 40 min) and 3 compared to the respective control group (remark EMF-Portal: the results for the other groups are presented ambiguously).
The authors conclude that exposure of dental pulp stem cells to 50 Hz magnetic fields might increase the cell viability.

Study character:

Study funded by

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