Study type: Medical/biological study (experimental study)

EMF frequency dependent differentiation of rat bone marrow mesenchymal stem cells to astrocyte cells. med./bio.

Published in: Neurosci Lett 2021; 744: 135587

Aim of study (acc. to author)

The effects of exposure of rat bone marrow-derived mesenchymal stem cells to different extremely low frequency magnetic fields on proliferation and differentiation into neurons and astrocytes should be investigated.

Background/further details

Rat bone marrow-derived mesenchymal stem cells were divided into the following groups: exposure to a sinusoidal magnetic field with 1) 25 Hz, 2) 50 Hz, 3) 75 Hz, exposure to a square wave magnetic field with 4) 25 Hz, 5) 50 Hz, 6) 75 Hz or 7) sham exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 25 Hz
Exposure duration: 1 hour/day for 7 days
Exposure 2: 50 Hz
Exposure duration: 1 hour/day for 7 days
Exposure 3: 75 Hz
Exposure duration: 1 hour/day for 7 days
Exposure 4: 25 Hz
Exposure duration: 1 hour/day for 7 days
Exposure 5: 50 Hz
Exposure duration: 1 hour/day for 7 days
Exposure 6: 75 Hz
Exposure duration: 1 hour/day for 7 days

Exposure 1

Main characteristics
Frequency 25 Hz
Type
Waveform
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Setup horizontal Helmholtz coil (300 turns, distance of 6 cm, and internal diameter of 16 cm) embedded in an open Plexiglas rectangular frame placed in a cell culture CO2 incubator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Exposure 3

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Exposure 4

Main characteristics
Frequency 25 Hz
Type
Waveform
  • rectangular
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Exposure 5

Main characteristics
Frequency 50 Hz
Type
Waveform
  • rectangular
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Exposure 6

Main characteristics
Frequency 75 Hz
Type
Waveform
  • rectangular
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 400 µT mean measured - -

Reference articles

  • Safari M et al. (2016): Proliferation and differentiation of rat bone marrow stem cells by 400 µT electromagnetic field.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell proliferation and cell viability were significantly decreased in group 1 (25 Hz sinusoidal), group 3 (75 Hz sinusoidal), group 5 (50 Hz square wave) and significantly increased in group 6 (75 Hz square wave) compared to the sham exposure. Cell differentiation into neurons and astrocytes was significantly increased in all exposure groups compared to the sham exposure. The largest increase in neuron differentiation was found in groups 2 (50 Hz sinusoidal) and 3 and the largest increase in astrocyte differentiation was found in groups 3 and 6.
The authors concluded that exposure of rat bone marrow-derived mesenchymal stem cells to extremely low frequency magnetic fields could inhibit proliferation and promote differentiation into neurons and astrocytes.

Study character:

Study funded by

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