Study type: Medical/biological study (experimental study)

The effects of radiofrequency fields on cell proliferation are non-thermal. med./bio.

Published in: Bioelectrochem Bioenerg 1999; 48 (1): 177-180

Aim of study (acc. to author)

In a previous study (publication 3775), it was shown that exposure of transformed human epithelial amnion cells (AMA) to a modulated radiofrequency field at 960 MHz at different power levels and exposure times, resulted in significant changes in cell proliferation.
In this study, the cells, the radiofrequency field, and the exposure time were the same, but the cells were exposed at a higher or lower temperature (39 or 35 +/- 0.1°C).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 960 MHz
Modulation type: pulsed
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 960 MHz
Type
Exposure duration continuous for 30 min
Modulation
Modulation type pulsed
Duty cycle 12 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional info

simulation of a GSM signal

Exposure setup
Exposure source
Chamber incubator
Setup The cells were seeded into the two central rows (#6 and #7) of sterile 96-well microtiter plates, each well containing a volume of 100 µl. One plate was placed in the TEM cell (powered or not) while the control plate was placed 20 cm away from it.
Additional info Static and AC (50 Hz) magnetic fields, measured with a three-axis fluxgate meter in both incubators did not exceed 1 µT.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 17 mV/cm peak value calculated - -
SAR 2.1 µW/g - calculated - in the cell culture

Reference articles

  • Kwee S et al. (1998): Changes in cell proliferation due to environmental non-ionizing radiation 2. Microwave radiation.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

There was at both temperatures a significant change in cell proliferation in the exposed cells in comparison to the control cells. On the other hand no significant change in proliferation rate was found in the sham-exposed cells at both temperatures. This shows that biological effects due to radiofrequency cannot be attributed only to a change of temperature.

Study character:

Study funded by

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