Study type: Medical/biological study (experimental study)

Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human mono mac 6 cells to radiofrequency radiation. med./bio.

Published in: Radiat Res 2006; 166 (3): 539-543

Aim of study (acc. to author)

To study the influence of radiofrequency electromagnetic fields in vitro on the cell cycle, BrdU uptake and the induction of apoptosis and necrosis of human Mono Mac 6 cells.

Background/further details

The positive controls were with gliotoxin or tetradecanoylphorbol acetate (PMA) treated cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Modulation type: pulsed
Exposure duration: continuous for 12 h
  • SAR: 2 W/kg unspecified

Exposure 1

Main characteristics
Frequency 1,800 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 12 h
Modulation
Modulation type pulsed
Additional info

GSM-DTX mode, with transmission reduced to 12 active frames per 104 frames, leading to a crest factor of 69. The frame structure results in 2, 8, and 217 Hz components. [Lantow et al., 2006]

Exposure setup
Exposure source
Chamber Two single-mode resonator cavities were placed inside a CO2 incubator that were randomly and blindly assigned for exposure [Schuderer et al., 2004].
Setup Six 35-mm Petri dishes were placed in each waveguide on a dish holder ensuring the correct placement in the H-field maxima of the standing waves.
Sham exposure A sham exposure was conducted.
Additional info Cells were treated simultaneously in the same incubator under the following conditions: untreated (sham or incubator control), RF exposed, sham exposed + PMA, RF exposed + PMA, sham exposed + gliotoxin, RF exposed + gliotoxin.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg unspecified unspecified - -

Reference articles

  • Lantow M et al. (2006): Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation.
  • Schuderer J et al. (2004): High Peak SAR Exposure Unit With Tight Exposure and Environmental Control for In Vitro Experiments at 1800 MHz

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after radiofrequency electromagnetic field exposure. In the positive control cells treated with gliotoxin and PMA, a significant increase in apoptotic and necrotic cells was seen.
Cell cycle analysis or BrdU incorporation for 72 h after exposure showed no differences between radiofrequency electromagnetic field- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G1 phase and a reduction in S-phase cells.

Study character:

Study funded by

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