Mice were randomly divided into four groups (n=50 per group): 1) static electric field exposure and 2) the respective control group, 3) 50 Hz electric field exposure and 4) the respective control group. The mice were exposed for 49 days and examined in the Morris water maze test on days 2-6, 9-13, 16-20, 30-34, and 44-48. After 7, 14, 21, 35, and 49 days of exposure, respectively ten mice per group were killed and the brain was taken to determine glutamine and GABA levels.
|Chamber||mice were housed and exposed in plastic cages (35 cm×25 cm×46 cm; length×width×height) with open tops; ten mice per cage|
|Setup||the two electrodes (3 m diameter) which generated the static electric field were arranged in parallel with a distance of 1 m; the exposed group was placed around the center of the bottom electrode; temperature in the laboratory was kept at 22°C ± 1°C and humidity at 40-50%|
|Additional info||cage of the control group was placed on the floor right under the exposure system|
|electric field strength||35 kV/m||-||measured||-||± 1.1 kV/m|
In the Morris water maze, no significant differences were found between the group exposed to the static electric field and the respective control group. However, in mice exposed to the 50 Hz electric field, the latency was significantly reduced on exposure day 33 compared to the respective control group.
The level of glutamine and GABA was not significantly changed in mice exposed to the static electric field compared to the control group. However, in mice exposed to the 50 Hz electric field, the glutamine level was significantly increased after 21 and 35 days of exposure in comparison to the control group.
The authors conclude that the results indicate that a static electric fields has no influence; however a 50 Hz electric field could improve learning and memory in mice.