Study type: Medical/biological study (experimental study)

The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma. med./bio.

Published in: Int J Mol Sci 2021; 22 (3): 1342

Aim of study (acc. to author)

The effects of exposure of different clear cell renal carcinoma cell lines and non-cancerous cells to a 50 Hz magnetic field on cell morphology and functions should be investigated.

Background/further details

786-O, 769-P and Caki1 cells were used as clear cell renal carcinoma cell lines, HEK293 as non-cancerous cell line for comparison. Cells from all cell lines were divided into an exposure group and a control group, respectively. Depending on the investigated parameter, cells were investigated directly, 24 hours, 48 hours and/or 72 hours after the end of exposure. Cells treated with hydrogen peroxide were used as a positive control to study oxidative stress.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 30 minutes/day for 5 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 30 minutes/day for 5 days
Exposure setup
Exposure source
Setup cells were seeded into flat-bottom 6-well plate and were cultured as monolayers; after 72 h of being plated, exposure at room temperature started; the magnetic field did not induce changes in the temperature of the plates or wells where the cells were located
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 4.5 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell morphology was not affected by exposure to the magnetic field in any cell line, while cell viability was slightly decreased in all cancer cells lines and increased in HEK203 cells compared to the control groups. Exposure to the magnetic field induced a G0/G1 arrest in cell cycle in all cancer cell lines compared to the control groups and a significant increase in apoptosis was observed in exposed 786-O cells compared to the control group. A significant increase in ROS generation was observed in exposure groups of all cell lines compared to the control groups. Cell migration and invasion abilities were significantly decreased in all exposed cancer cells compared to the control groups.
The authors concluded that exposure to a 50 Hz magnetic field might have different effects on clear cell renal carcinoma cell lines and non-cancerous cells.

Study character:

Study funded by

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