The cells were exposed to various intensities and durations of 60 Hz sinusoidal magnetic field in combination with various concentrations of serum, caspase-3 inhibitor, or N-acetyl-L-cysteine (as antioxidant) in the media.
In the three prostate cancer cells, magnetic field exposure inhibited cell growth and significantly promoted apoptosis and cell cycle arrest proportional to the intensity and duration of the magnetic field. Cleaved caspase-3, and reactive oxygen species increased. Pretreatment with a caspase-3 inhibitor or the antioxidant N-acetyl-L-cysteine significantly attenuated magnetic field induced cell growth inhibition and cell death. Media replacement experiments failed to show any notable change in the magnetic field effects.
The data suggest that a possibility of using 60 Hz magnetic field in radiation therapy of prostate cancer could usefully be investigated.