Study type: Medical/biological study (experimental study)

A 60-Hz sinusoidal magnetic field induces apoptosis of prostate cancer cells through reactive oxygen species. med./bio.

Published in: Int J Radiat Biol 2008; 84 (11): 945-955

Aim of study (acc. to author)

To study the effects of power frequency magnetic fields on cell growth in different prostate cancer cell lines and the underlying mechanisms for these effects.

Background/further details

The cells were exposed to various intensities and durations of 60 Hz sinusoidal magnetic field in combination with various concentrations of serum, caspase-3 inhibitor, or N-acetyl-L-cysteine (as antioxidant) in the media.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 24 h for serveral days - possibly up to 4 days - not exactly stated

General information

In some experimental sets, a certain percentage of serum, Caspase-3 inhibitor, or NAC at various concentrations was added to the serum-free medium 1h or 2 h before MF exposure.

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 24 h for serveral days - possibly up to 4 days - not exactly stated
Exposure setup
Exposure source
Setup 28 cm x 28 cm x 30 cm rectangular Helmholtz coils on open acrylic frames with three bundles (2000 turns per bundle) of 0.5 mm copper wire, aligned parallel, 20 cm apart
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.2 mT minimum measured - -
magnetic flux density 0.5 mT - measured - -
magnetic flux density 1 mT - measured - -
magnetic flux density 2.5 mT maximum measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the three prostate cancer cells, magnetic field exposure inhibited cell growth and significantly promoted apoptosis and cell cycle arrest proportional to the intensity and duration of the magnetic field. Cleaved caspase-3, and reactive oxygen species increased. Pretreatment with a caspase-3 inhibitor or the antioxidant N-acetyl-L-cysteine significantly attenuated magnetic field induced cell growth inhibition and cell death. Media replacement experiments failed to show any notable change in the magnetic field effects.
The data suggest that a possibility of using 60 Hz magnetic field in radiation therapy of prostate cancer could usefully be investigated.

Study character:

Study funded by

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