Study type: Medical/biological study (experimental study)

The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro. med./bio.

Published in: Prz. Elektrotechniczny 2017; 1 (1): 161–164

Aim of study (acc. to author)

The effects of exposure of normal cells and cancer cells to a 50 Hz magnetic field on cell viability should be investigated.

Background/further details

Human osteosarcoma cells and human fibroblast cells were both exposed to magnetic fields with different combinations of magnetic flux density (2 mT, 2.5 mT, 3 mT, 4 mT, 6 mT and sham exposure) and exposure duration (30 minutes, 1 hour, 2 hours, 3 hours).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 30 minutes - 3 hours
Exposure 2: 50 Hz
Exposure duration: 30 minutes - 3 hours
Exposure 3: 50 Hz
Exposure duration: 30 minutes - 3 hours
Exposure 4: 50 Hz
Exposure duration: 30 minutes - 3 hours
Exposure 5: 50 Hz
Exposure duration: 30 minutes - 3 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes - 3 hours
Exposure setup
Exposure source
  • not specified
  • Magneris generator (Astar)
Chamber 96 well plates
Setup two-piece flat applicator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes - 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2.5 mT - measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes - 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT - measured - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes - 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 4 mT - measured - -

Exposure 5

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes - 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 6 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In all exposure groups of the osteosarcoma cells except in exposure for 30 minutes, exposure to the magnetic field resulted in a significantly decreased cell viability compared to sham exposure. In fibroblasts, exposure to the magnetic field led to a significantly increased cell viability in all exposure groups compared to the sham exposure.
The authors conclude that a 50 Hz magnetic field exposure might have different effects on cell viability in normal cells compared to cancer cells.

Study character:

Study funded by

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