The RF generator was connected to a high Q coupling system consisting of a transmitting (Tx) and a receiving (Rx) head with adjustable distance mounted on a swivel bracket, allowing the RF field to be oriented horizontally or vertically. A focused electromagnetic field was produced with a useful diameter of 30 cm and with a peak intensity at 7 cm from the central axis of the Tx and Rx heads.
Aufbau
Five different concentrations of functionalized single-walled carbon nanotubes (SWNTs, 5 mg/l, 50 mg/l, 125 mg/l, 250 mg/l, and 500 mg/l) were diluted in deionised water and placed in a 1.5-ml circular quartz cuvette located between the Tx and Rx heads (7.5 cm apart). The samples were located at the mid plane of the working volume.
Zusatzinfo
Temperature in the SWNT solutions, in solutions without SWNTs, and in water alone were measured continuously before, during, and for 2 minutes after the RF field activation.
Cells were placed individually on a Teflon holder between the Tx and Rx heads that were oriented vertically and 7.5 cm apart. Media containing various concentrations of SWNTs, solution without SWNTs, or media alone were added to the cells grown to near confluence.
Animals were placed individually on a platform between the Tx and Rx heads that were oriented vertically and 10 cm apart. Each animal was positioned so the hepatic tumour was located at the mid plane of the working volume.
Zusatzinfo
An intrahepatic tumour introduced in the rabbits was injected directly with either a solution of SWNTs (500 mg/l) or with a control solution (no SWNTs). A control group obtained an injection of SWNTs but was not treated with RF. A final control group received RF exposure without injection of SWNTs or control solution.
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